An improved and low-cost protocol for high-quality DNA isolation for the Chagas disease vectors.

An improved and low-cost protocol for high-quality DNA isolation for the Chagas disease vectors. Infect Genet Evol. 2020 Jan 20;:104201 Authors: Flores DDC, Nava-García E, Arellano E Abstract An improved protocol for DNA extraction for the Chagas disease vectors is proposed based on modification to a low cost method described twenty years ago. Quality DNA and high molecular weight were obtained from all samples. NADH-4 gene was successfully amplified by PCR using the isolated DNA. The extraction protocol presented in this technical note is a fast, low-cost, and non-aggressive method to human health for obtaining genetic data from this group of epidemiological importance and potentially other insects. PMID: 31972328 [PubMed - as supplied by publisher]
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research