Quantitation of CMV Specific T-Cell Expansion Using T Cell Receptor Beta Locus Deep Sequencing to Identify Patients at Risk of Viral Complications

An effective T cell response is necessary for the control of cytomegalovirus (CMV) reactivation after allogeneic stem cell transplant (alloBMT) - CMV is associated with significant morbidity, especially in patients (pts) who require recurrent episodes of antiviral therapy& those who develop associated end organ dysfunction (CMV disease). We sought to determine whether deep sequencing of T cell receptor beta (TRB) loci could identify& quantitate T cell clonotypes with specificity for CMV epitopes in the early post-alloBMT period and assess association with virus related outcomes.

Source: Biology of Blood and Marrow Transplantation - January 24, 2020 Category: Hematology Authors: James Anton Kuzich, Yamuna Kankanige, Jerick Guinto, Georgina Ryland, Michelle McBean, Ella Thompson, Eric Wong, Rachel Koldej, Jenny Collins, David Westerman, David Stuart Ritchie, Piers Blombery Tags: 475 Source Type: research