Influence mechanism of miRNA-144 on proliferation and apoptosis of osteosarcoma cells.

Influence mechanism of miRNA-144 on proliferation and apoptosis of osteosarcoma cells. Oncol Lett. 2020 Feb;19(2):1530-1536 Authors: Zhang X, Li Z, Ji W, Chen X, Gao Q, Li D, Qin H Abstract Influence mechanism of miRNA-144 on proliferation and apoptosis of osteosarcoma cells was investigated. A total of 51 cases of osteosarcoma tissue samples were collected in the department of orthopedic surgery, Xuzhou Children's Hospital, Xuzhou Medical University from January 2014 to February 2017. Additionally, 48 cases of normal bone tissues were collected. qRT-PCR was used to detect the expression of miR-144. Correlation of miR-144 expression in serum and cancer tissues was detected. ROC curve was drawn to analyze the diagnostic value of miR-144 in patients with osteosarcoma. CCK-8 was used to detect the effect of miR-144 on the proliferation ability of U2-OS after transfection. The ratio of U2-OS apoptosis after miR-144 transfection was detected by flow cytometry. Western blot analysis was used to detect the expression of Bax, caspase-3 and Bcl-2 proteins in U2-OS after transfection. The relative expression of miR-144 in osteosarcoma and osteosarcoma serum was significantly lower than that in normal bone tissue and normal human serum (P<0.05). Serum in patients was positively correlated with the expression of miR-144 in cancer tissues. The area under the miR-144 curve was 0.852, 95% CI, 0.768-0.936. The relative expression of miR-144 in MG...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research