Novel fluorescence palladium-alkoxime complexes: Synthesis, characterization, DNA/BSA spectroscopic and docking studies, evaluation of cytotoxicity and DNA cleavage mechanism

Publication date: 15 April 2020Source: Journal of Molecular Structure, Volume 1206Author(s): Kazem Karami, Nasrin Jamshidian, Atefe Bagheri, Afsaneh Hajiaghasi, Amir Abbas Momtazi-Borojeni, Elham Abdollahi, Azar Shahpiri, Negar Azizi, Janusz LipkowskiAbstractThe new alkoxime ligand (E)-anthracene-9-carbaldehyde-O-methyl oxime (1) derivative of 9-anthraldehyde and its palladium (II) complexes trans-Pd(C15H10NOCH3)2(OAc)2 (2) and [cis-Pd(C15H10NOCH3)(Py)2(OAc)](OAc) (3) were synthesized and characterized by spectroscopic techniques. Also, the ligand (1) structure was characterized by X-ray crystallography. Then their interaction with calf thymus deoxyribonucleic acid (CT-DNA) and bovine serum albumin (BSA) was explored using absorption and emission spectral methods. The inner filter effect was corrected to get accurate data. Ligand (1) and complex (3) bind strongly to DNA via intercalation and complex (2) binds to it by groove binding mode. The binding modes were further confirmed by CD spectra. BSA binding aspects of synthesized compounds have been studied through various biophysical techniques, and the efficiency of energy transfer between them and BSA has been calculated according to Förster energy transfer theory (FRET). Furthermore, they were assessed for in vitro cytotoxicity testing on the colon carcinoma (CT26) and breast cancer (4T1) cell lines. The three compounds displayed better cytotoxic profiles against CT26 than 4T1 cancer cell lines. Also, their abilit...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research

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CONCLUSION: The complexes showed noticeable cytotoxicity and stand as promising antitumor candidates for further application. PMID: 32067614 [PubMed - as supplied by publisher]
Source: Combinatorial Chemistry and High Throughput Screening - Category: Chemistry Authors: Tags: Comb Chem High Throughput Screen Source Type: research
Planta Med DOI: 10.1055/a-1091-8831Three new (alterchothecenes A – C, 1 –3) and 3 known (4 –6) trichothecenes, along with 9 known compounds (7 –15), were isolated from the culture of Alternaria sp. sb23, an endophytic fungus separated from the root of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of 1–3 were determined through comparison of the experimental electronic circular dichroism (ECD) spectra and optical rotations with similar analogues. In vitro cytotoxicity tests of compounds 1–6 agai...
Source: Planta Medica - Category: Drugs & Pharmacology Authors: Tags: Original Papers Source Type: research
In conclusion, OLEO represents a natural product effective in reducing the glycolytic metabolism of different tumor types, revealing an extended metabolic inhibitory activity that may be well suited in a complementary anti-cancer therapy.
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Publication date: Available online 24 January 2020Source: Arabian Journal of ChemistryAuthor(s): Fabiana L. Silva, Raquel V.S. Silva, Paola C. Branco, Letícia V. Costa-Lotufo, Cynthia Murakami, Maria C.M. Young, Débora A. Azevedo, Paulo R.H. MorenoAbstractCinnamomum stenophyllum (Meisn.) Vattimo-Gil (Lauraceae) is a native and vulnerable Brazilian species restricted to the Atlantic Forest. The leaf essential oil obtained by hydrodistillation was characterized for the first time by two-dimensional gas chromatography with time-of-flight mass spectrometry (GC×GC-TOFMS). This analysis resulted in the tentat...
Source: Arabian Journal of Chemistry - Category: Chemistry Source Type: research
Targeted therapy using monoclonal antibodies conjugated to toxins is gaining space in the treatment of cancer. Here, we report the anti-tumor effect of a new antibody drug conjugate (ADC) delivering a HDAC inhibitor to ErbB2+ solid tumors. Trastuzumab was partially reduced with tris [2-carboxyethyl] phosphine (TCEP) and conjugated to ST7464AA1, the active form of the prodrug HDAC inhibitor ST7612AA1, through a maleimide-thiol linker to obtain the Antibody Drug Conjugate (ADC) ST8176AA1. The average drug/antibody ratio (DAR) was 4.5 as measured by hydrophobic interaction chromatography (HIC). Binding of ST8176AA1 to ErbB2 r...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 3 January 2020Source: Journal of Organometallic ChemistryAuthor(s): T.A. Antonenko, D.B. Shpakovsky, D.А. Berseneva, Yu.A. Gracheva, L.G. Dubova, P.N. Shevtsov, O.M. Redkozubova, E.F. Shevtsova, V.A. Tafeenko, L.A. Aslanov, E.R. MilaevaAbstractTwo series of organotin(IV) carboxylates based on phenolic antioxidants RCOOH and R(CH2)2COOH (R = 3,5-di-tert-butyl-4-hydroxyphenyl) and natural bile (cholanic) acids of formulae (RCOO)2SnMe2 (1); (RCOO)2SnBu2 (2); (R(CH2)2COO)2SnMe2 (3); (R(CH2)2COO)2SnBu2 (4); (cholate)SnPh3 (5); (deoxycholate)SnPh3 (6); (lithocholate)SnPh3 (7); (cholate)SnM...
Source: Journal of Organometallic Chemistry - Category: Chemistry Source Type: research
In this study, in vitro/in vivo expression, biomarker potential and genetic/epigenetic alterations of DNAJC10 were analyzed in breast cancer. METHODS: Real-time qRT-PCR and immunohistochemistry methods were used to determine the expression level of DNAJC10 gene in breast cancer cell lines and clinical samples. The Kaplan-Meier plotter was used to evaluate the survival prognostic value of DNAJC10 mRNA expression in breast cancer patients. Mutation screening software and methylation-specific PCR were used to screen genetic alterations and methylation status of DNAJC10 promoter regions, respectively. RESULTS: DNAJC1...
Source: Breast Cancer - Category: Cancer & Oncology Authors: Tags: Breast Cancer Source Type: research
Abstract Immunogenic cell death (ICD) occurs when a dying cell releases cytokines and damage-associated molecular patterns, acting as adjuvants, and expresses Ags that induce a specific antitumor immune response. ICD is studied mainly in the context of regulated cell death pathways, especially caspase-mediated apoptosis marked by endoplasmic reticulum stress and calreticulin exposure and, more recently, also in relation to receptor-interacting protein kinase-driven necroptosis, whereas unregulated cell death like accidental necrosis is nonimmunogenic. Importantly, the murine cancer cell lines used in ICD studies o...
Source: Journal of Immunology - Category: Allergy & Immunology Authors: Tags: J Immunol Source Type: research
Conclusion: According to these results, MACC1 expression may be a marker of breast carcinoma as well as an independent predictor of poor prognosis. In addition, MACC1 may not affect angiogenesis in breast cancer or even if it has an effect, it may not be associated with VEGF. However, it would be appropriate to support these results in a larger series by investigating in vivo and in vitro studies. PMID: 32031117 [PubMed - in process]
Source: Indian Journal of Pathology and Microbiology - Category: Pathology Authors: Tags: Indian J Pathol Microbiol Source Type: research
In conclusion, significant antitumor activity and weak toxicity were recorded for this hybrid compound, characterizing it as a potential anticancer compound.
Source: Molecules - Category: Chemistry Authors: Tags: Communication Source Type: research
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