Facilitating the enzymatic conversion of lysine to cadaverine in engineered Escherichia coli with metabolic regulation by genes deletion

In this study, nine different types of deletion strains were constructed from the aforementioned four genes to explore their effects on cadaverine production in a T7 promoter based systemic bicistronic cadA-cadB transformed recombinant E. coli. Results show that all the deletion strains could increase cadaverine productivity compared to the deletion-free strain when the culture was supplemented with L-lysine. The cadaverine production from L-lysine with the deletion strains increased 3 folds when compared with that of the deletion-free strain at the cultivation time of 16 h. This indicates that the deletion strain could successfully facilitate lysine consumption and block the undesirable metabolic pathways.Graphical abstract
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research