GSE144101 High-throughput sequencing analysis of a “hit and run” cell and animal model of KSHV tumorigenesis.

Contributors : E A Mesri ; J Naipauer ; M C AbbaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusKaposi's sarcoma (KS), is an AIDS-associated neoplasm caused by the KS herpesvirus (KSHV). A mouse model of KSHV-dependent tumorigenicity, allowed us to induce KSHV viral-episome loss following tumor development to test the plausibility of “hit and run” mechanism by KSHV. RNA-seq-transcriptome analysis and CpG-methylation were performed on KSHV positive cells, KSHV positive tumors and tumors that developed following viral-episome loss. During KSHV tumorigenesis, hypo-methylation was detected of oncogenic and differentiation pathwa ys. In contrast, during tumorigenesis following KSHV-episome loss, a tendency towards hyper-methylation was detected. We found the same set of innate-immunity related mutations undetected in KSHV-infected cells but present in all KSHV-positive tumors, indicating that pre-existing host mutations that provide an in vivo growth advantage are clonally-selected and contribute to KSHV-tumorigenesis. We found de novo mutations related to cell proliferation that, together with the PDGFRAD842V, were responsible for driving tumorigenesis in absence of the KSHV-episomes. Virally-induced irreversible gene tic and epigenetic oncogenic alteration supports the possibility of “hit and run” KSHV-sarcomagenesis consistent with the existence of LANA-negative spindle-cells in KS lesions.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

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Publication date: Available online 10 February 2020Source: IDCasesAuthor(s): Naoki Kawakami, Ho Namkoong, Masayuki Shimoda, Hiroshi Kotani, Hiroshi Fujiwara, Naoki Hasegawa
Source: IDCases - Category: Infectious Diseases Source Type: research
The objective of this research was to estimate the incidence of malignancies in HIV patients and their surveillance. Age and sex-adjusted incidence was compared to the incidence calculated by the Spanish Cancer Registry network (REDECAN) in 2015. RESULTS: We included 2,773 patients (41,238 patients-year). Two hundred and eleven malignancies were diagnosed in 182 patients. Non-AIDS defining cancers accounted for 78.2% of the malignancies. The global incidence of cancer was 485 cases per 100,000 person-years. Twenty-year mortality rate was 31.2% in patients with cancer and 7.8% in patients without cancer. In men, adjuste...
Source: Medicina Clinica - Category: General Medicine Tags: Med Clin (Barc) Source Type: research
ConclusionCollectively, similar variants of ORF-73 markers involved in the immune response may interact with targeted host proteins as predicted by our computational analysis. This work also suggests the existence of potential conformational changes that need to be further explored to help elucidate the role of immune signaling during KS towards the development of therapeutic applications.
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
oêlho MRCD Abstract Human gammaherpesvirus 8 (HHV-8) is the etiologic agent of Kaposi's sarcoma (KS), one of the most common cancers in people living with HIV/AIDS. It is believe that the course of both HIV and HHV-8 infection is associated with the imbalance of anti- and/or pro-inflammatory cytokines. Here, we evaluated the IL-6, TNF-α, IL-10, CCL2 and CXCL10 serum concentrations in HIV- and HIV/HHV-8 (without KS) individuals, and in patients with cutaneous or visceral AIDS-KS. Serum concentrations of IL-6, IL-10 and CXCL10 were significantly higher in the AIDS-KS group compared to HIV and HIV/HHV-8 i...
Source: Human Immunology - Category: Allergy & Immunology Authors: Tags: Hum Immunol Source Type: research
Acquired immunodeficiency syndrome (AIDS) is a chronic systemic disease. This condition can be manifested if the patient is infected by the human immunodeficiency virus (HIV). This virus attacks the immune system and interferes in the host resistance. Consequently, infected organisms become immunosuppressed and susceptible to acquiring other opportunistic infections and injuries. The HIV-related diseases most frequent in the oral cavity are candidiasis, gingivitis, oral hairy leukoplakia, herpes simplex, and Kaposi sarcoma.
Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics - Category: ENT & OMF Authors: Source Type: research
AIDS associated with Kaposi sarcoma (KS-AIDS) is one of the most prevalent malignant neoplasms in people with HIV. It usually presents initially as violaceous cutaneous lesions, but oral mucosa, lymph nodes, and visceral organs may be affected, sometimes without skin involvement, being an important prognostic factor. The aim of this presentation is to report 4 KS-AIDS cases with oral involvement. All cases occurred in black or mixed-race men, aged between 23 and 31, showing extremely low CD4 counts (range, 5-132 cells/mm ³) and high HIV viral load.
Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics - Category: ENT & OMF Authors: Source Type: research
Kaposi sarcoma (KS) is a malignant vascular neoplasia with occurrence in 15% to 20% of patients with AIDS. It presents multiple macules and purple papules on the skin. Treatment depends on subtype and disease stage, with variable prognosis. Oral lesions are rare. A 31-year-old male patient was attended with a 6-month painless, erythematous, exophytic, bleeding lesion with variable surface texture located in the hard palate, soft palate, and alveolar ridge. He also presented skin patches, left eye lesion, and weight loss of 20 kg in 2 months.
Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics - Category: ENT & OMF Authors: Source Type: research
Although the Kaposi sarcoma (KS) is one of the AIDS defining entity and seen in almost one third of HIV infected patients with low CD4 cell counts, it is not uncommon in HIV seronegative persons, but genital K...
Source: Infectious Agents and Cancer - Category: Cancer & Oncology Authors: Tags: Short Report Source Type: research
AbstractThe development of anti-N-methyl-D-aspartate (NMDA) receptor encephalitis is often associated with neoplasia or infectious diseases as antibodies against neurons or synaptic proteins surface. A 30-year-old male patient was admitted to our department because of neurocognitive symptoms, particularly memory difficulties which had appeared a year prior and since then had been increasing. He had a medical history of smoking and hypertension. On examination, there were no focal neurological deficits. However, neuropsychological tests confirmed a lack of concentration and short-term memory impairment. Brain magnetic reson...
Source: Journal of NeuroVirology - Category: Neurology Source Type: research
CONCLUSION: Bortezomib is well-tolerated and active in AIDS-KS. The 60% PR rate is notable given the dose-finding nature of the study in a r/r population. Changes in KSHV PDCN and HIV VL trended as hypothesized. PMID: 31624104 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Clin Cancer Res Source Type: research
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