Antimicrobial susceptibility of Trueperella pyogenes isolated from food-producing ruminants

Publication date: Available online 22 January 2020Source: Veterinary MicrobiologyAuthor(s): Ángela Galán-Relaño, Lidia Gómez-Gascón, Belén Barrero-Domínguez, Inmaculada Luque, Francisco Jurado-Martos, Ana I. Vela, Celia Sanz-Tejero, Carmen TarradasAbstractA total of 96 Trueperella pyogenes isolates, an opportunistic pathogen of food-producing ruminants, obtained from cattle (n = 34), sheep (n = 35) and goats (n = 27), and identified by Real Time PCR (qPCR), were analysed to determine the susceptibility to 12 antimicrobials commonly used in livestock, using a broth microdilution. The Minimal Inhibitory Concentration (MIC) distribution was unimodal for half of the antimicrobials tested with the exception of apramycin, gentamicin, streptomycin, oxytetracycline, tylosin, and erythromycin all of which showed bimodal MIC distributions. Low MIC90 values for penicillin, amoxicillin, ceftiofur, enrofloxacin, and gentamicin (<1 μg/ml) were obtained, suggesting that these antimicrobials would be the most effective first line empiric treatment for T. pyogenes infections in livestock. Furthermore, according to the specific T. pyogenes breakpoints for penicillin, sulfamethoxazole/trimethoprim and erythromycin, 93.7% of isolates were susceptible to penicillin and 77.2% to erythromycin, whereas 92.7% were non-susceptible to sulfamethoxazole/trimethoprim. Significant differences were observed in the MIC distribution of almost all antimicrobials, except enrofloxacin, ...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research