Kinetics aspects of Gamma-hydroxybutyrate dehydrogenase

Publication date: Available online 22 January 2020Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Esther S. Taxon, Lila P. Halbers, Stanley M. ParsonsAbstractTwo groups of metabolically related enzymes, the Group III family of Fe2+-dependent alcohol dehydrogenases (ADHs) and the separate subfamily of nucleoside diphosphates linked to x (nudix) hydrolases that activate Group III ADHs are under-characterized. Here we report the steady-state initial-velocity forward direction (alcohol → aldehyde) reaction of a Group III ADH, namely gamma-hydroxybutyrate dehydrogenase (GHBDH, UniProt: Q59104), cloned from Cupriavidus necator as a fusion protein. We also report the effects of nudix hydrolases on the GHBDH reaction. At optimal pH 9.0, the GHBDH reaction is activated ~2-fold by two different saturating purified nudix hydrolases, namely Bacillus methanolicus activator (ACT, UniProt: I3EA59) and Escherichia coli NudF (UniProt Q93K97) proteins. At physiological pH values of ~7.0, ACT activates by>3.5-fold. Initial-rate characterization at pH 9.0 of the forward direction un-activated and ACT-activated reactions show for both cases competitive inhibition by the product succinic semialdehyde versus GHB, and noncompetitive inhibitions by the three other substrate-product combinations. This pattern is consistent with NAD+ binding first in Mono-Iso Theorell-Chance kinetics. Mutants of some possibly important residues in GHBDH also were characterize...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - Category: Biochemistry Source Type: research

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Publication date: Available online 20 February 2020Source: Carbohydrate PolymersAuthor(s): Shivayogi S. Narasagoudr, Veena G. Hegde, Vinayak N. Vanjeri, Ravindra B. Chougale, Saraswati P. MastiAbstractEthyl vanillin (EV) incorporated chitosan (CS)/poly(vinyl alcohol)(PVA) blend films of various ratios (1:3, 1:1 and 3:1) were prepared by solvent casting technique. The effect of EV on the mechanical, structural, barrier, optical, food compatibility and antibacterial properties of the CS/PVA films were investigated. Mechanical properties showed that addition of EV increased tensile strength of CPEV-1, CPEV-2 and CPEV-3 films ...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Molecules, Vol. 25, Pages 931: Stereoselective Bioreduction of α-diazo-β-keto Esters Molecules doi: 10.3390/molecules25040931 Authors: González-Granda Costin Sá Gotor-Fernández Diazo compounds are versatile reagents in chemical synthesis and biology due to the tunable reactivity of the diazo functionality and its compatibility with living systems. Much effort has been made in recent years to explore their accessibility and synthetic potential; however, their preparation through stereoselective enzymatic asymmetric synthesis has been scarcely reported in the literature. Alco...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
In this study, biocomposite films based on polyvinyl alcohol (PVA), gum arabic (GA) and chitosan (CS) incorporated with BPEO and GEO were fabricated by solvent casting method. FTIR, XRD, SEM and DSC were performed with mechanical and antimicrobial properties of PVA/GA/CS films with and without BPEO and GEO. The BPEO-PVA/GA/CS film showed heterogeneous rough surface with cavities containing entrapment of BPEO droplets, whereas, the GEO-PVA/GA/CS film showed heterogeneous rough surface with coarseness due to the incorporation of respective EOs. The BPEO and GEO incorporated PVA/GA/CS films were considerable resistant to brea...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
Conclusion: The present investigations have exposed that the myrtle berries:cloves, myrtle berries: cinnamon:cloves extracts could be used in traditional medicine as natural antimicrobial agents in treatment the bacterial infections.
Source: Current Nutrition and Food Science - Category: Nutrition Source Type: research
ConclusionHandrubbing with sprayed ABHR was non-inferior to rubbing with ABHR poured onto a hand palm to reduce bacterial counts on hands under experimental conditions. Rubbing with sprayed ABHR may be an acceptable alternative hand hygiene method pending assessment in other settings and for other pathogens.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Conclusion: The ability of E. senticosus root and bark extracts to promote polarization of human macrophages towards anti-inflammatory M2a and M2b phenotypes, respectively, might underlay the immunoregulatory activities and point to potential wound healing promoting effects of this medicinal plant.Graphical abstract
Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
Abstract 1-Cyanocyclohexaneacetic acid (1-CHAA) is a critical intermediate for the synthesis of the antiepileptic agent gabapentin. Previously, our group has established a novel manufacturing route for 1-CHAA through bioconversion catalyzed by an Escherichia coli (E. coli) nitrilase whole cell catalyst. However, the nitrilase expressed in E. coli has several drawbacks such as a low level of reusability, which hampered its industrial application. Herein, we investigated the potential of using the methylotrophic yeast Pichia pastoris (P. pastoris) for producing the nitrilase whole cell catalyst. To achieve strains w...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
Abstract Benzyl alcohol is a naturally occurring aromatic alcohol and has been widely used in the cosmetics and flavor/fragrance industries. The whole-cell biotransformation for synthesis of benzyl alcohol directly from bio-based L-phenylalanine (L-Phe) was herein explored using an artificial enzyme cascade in Escherichia coli. Benzaldehyde was first produced from L-Phe via four heterologous enzymatic steps that comprises L-amino acid deaminase (LAAD), hydroxymandelate synthase (HmaS), (S)-mandelate dehydrogenase (SMDH) and benzoylformate decarboxylase (BFD). The subsequent reduction of benzaldehyde to benzyl...
Source: Chemistry, an Asian Journal - Category: Chemistry Authors: Tags: Chem Asian J Source Type: research
Conclusions: This study demonstrated the possibility of using ethanol as the carbon source by engineered E. coli strains. It can serve as the basis for the construction of more robust strains in the future though the catabolic capacity of ethanol should be further improved. PMID: 31993378 [PubMed]
Source: Cell Research - Category: Cytology Authors: Tags: J Biol Res (Thessalon) Source Type: research
Publication date: 2020Source: Toxicology Reports, Volume 7Author(s): Afaf M. Weli, Hanady S. Al-Saadi, Rouqaya S. Al-Fudhaili, Amzad Hossain, Zabida Binti Putit, Mahmood K. JasimAbstractMedicinal plant as herbal medicine is widely used to cure infectious diseases. Rubus fruticosus L (R. fruticosus) has been studied for its antimicrobial and cytotoxic activities. Different polarity leave extracts were prepared by using hexane, chloroform, ethyl acetate and hydro alcoholic solvents. Agar diffusion method has been used to assess the antibacterial activity against two gram-positive Enterococcus faecalis and Staphylococcus aure...
Source: Toxicology Reports - Category: Toxicology Source Type: research
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