Straightening Out the Elasticity of Myosin Cross-Bridges.

Straightening Out the Elasticity of Myosin Cross-Bridges.
Biophys J. 2020 Jan 13;:
Authors: Linari M, Piazzesi G, Pertici I, Dantzig JA, Goldman YE, Lombardi V
Abstract
In a contracting muscle, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward the center of the sarcomere, the structural unit of striated muscle. Cross-bridge attachments in the sarcomere have been reported to exhibit a similar stiffness under both positive and negative forces. However, in vitro measurements on filaments with a sparse complement of heads detected a decrease of the cross-bridge stiffness at negative forces attributed to the buckling of the subfragment 2 tail portion. Here, we review some old and new data that confirm that cross-bridge stiffness is nearly linear in the muscle filament lattice. The implications of high myosin stiffness at positive and negative strains are considered in muscle fibers and in nonmuscle intracellular cargo transport.
PMID: 31968230 [PubMed - as supplied by publisher]

Source: Biophysical Journal - January 12, 2020 Category: Physics Authors: Linari M, Piazzesi G, Pertici I, Dantzig JA, Goldman YE, Lombardi V Tags: Biophys J Source Type: research