A peppermint oil emulsion stabilized by resveratrol-zein-pectin complex particles: Enhancing the chemical stability and antimicrobial activity in combination with the synergistic effect

In this study, peppermint oil and resveratrol display synergistic effect against the Gram-positive bacteria Staphylococcus aureus and the Gram-negative bacteria Salmonella Typhimurium. Partially wettable resveratrol-loaded zein-pectin complex particles with a three-phase contact angle of ∼78° were fabricated via a desolvation method. Peppermint oil emulsions with the co-inclusion of resveratrol were successfully prepared by zein-pectin complex particles, showing a high encapsulation efficiency for peppermint oil (∼88%) and resveratrol (∼99%). Addition of pectin decreased size distribution of the emulsions, improved antimicrobial activity, physical and chemical stability and prolonged antimicrobial efficiency against Staphylococcus aureus and Salmonella Typhimurium. Overall, the current study may have a valuable contribution to develop an efficient antimicrobial system based on the synergistic effect of combined agents and a single emulsion stabilized by protein-polysaccharide complex particles.Graphical abstract
Source: Food Hydrocolloids - Category: Food Science Source Type: research

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It is well-established that the spread of many multidrug-resistant (MDR) bacteria is predominantly clonal. Interestingly the international clones/sequence types (STs) of most pathogens emerged and disseminated during the last three decades. Strong experimental evidence from multiple laboratories indicate that diverse fitness cost associated with high-level resistance to fluoroquinolones contributed to the selection and promotion of the international clones/STs of hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA), extended-spectrum β-lactamase-(ESBL)-producing Klebsiella pneumoniae, ESBL-produci...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 20 February 2020Source: Materials Today: ProceedingsAuthor(s): Asha Gangadharan, Jismy Jolly, Nayomi JohnAbstractBioprospecting is the searching of novel bioactive natural products from unexplored natural environment. The aim of the study was to isolate novel therapeutic agents from marine bacterium. The marine bacterium was identified as Serratia marcescens using molecular identification methods. The secondary metabolites were extracted and showed single spot on TLC at Rf of 0.74. The metabolite showed good antioxidant and antibacterial activity against Staphylococcus aureus, pseudomonas...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
Publication date: Available online 19 February 2020Source: Materials Chemistry and PhysicsAuthor(s): Natasha Vieira Sievers, Liliane Damaris Pollo, Gertrudes Corção, Nilo Sérgio Medeiros CardozoAbstractThis work reports a new method to obtain polypropylene/titanium dioxide (PP/TiO2) films with antibacterial activity through the in situ synthesis of nanostructured TiO2 directly in the polymer solution. In order to compare the performance of the different types of particles, TiO2 particles were also prepared utilizing the sol-gel method and subsequently incorporated into the polymer to form PP/TiO2 films...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
In this study we designed and synthetized various derivatives of NCR247, including shorter fragments and various chimeric derivatives. The antimicrobial activity of these peptides was tested on the ESKAPE bacteria; Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli as a member of Enterobacteriaceae and in addition Listeria monocytogenes and Salmonella enterica. The 12 amino acid long C-terminal half of NCR247, NCR247C partially retained the antimicrobial activity and preserved the multitarget interactions with partners of NCR247. Nevert...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, unlike traditional alheiras which often contain pathogenic agents, no harmful organisms nor chemical hazards were found in these new products, even though produced by the same companies.
Source: Food Control - Category: Food Science Source Type: research
The objective of this study was to isolate indigenous probiotic organisms and assess their probiotic activity and therapeutic characteristics. The isolates were identified as Lactobacillus fermentum (isolates 2, 4, 6, 7, 8, and 9), Lactobacillus salivarius (isolate 13), and Lactobacillus plantarum (isolates 32 and 36). Five isolates showed growth at pH 2.5, while all isolates could grow at pH 8.5. All isolates showed good growth upto 5% NaCl concentration while two isolates showed growth in 7% NaCl concentration. All the isolates were susceptible to most of the broad-spectrum antibiotics. Cell-free suspensions from the iso...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
This study evaluates the application of thermosonication for the inactivation of pathogenic microorganisms, and its effect on antioxidant activity and retinol level in donated human milk. We evaluate the effect of thermosonication on Staphylococcus aureus, Escherichia coli, and Salmonella spp. microorganisms in human milk by analyzing the effect of time and temperature of the thermosonication on these microorganisms using a central composite rotatable design. Also evaluated the effects of thermosonication (60 °C/4 min) on antioxidant activity using DPHH (2, 2-diphenyl-1-picrylhydrazyl) free radical and ABTS (2,2&pr...
Source: Food Control - Category: Food Science Source Type: research
Authors: Sbhatu DB, Abraha HB Abstract Medicinal plants and plant remedies have been in use in Ethiopia for centuries. Studies on ethnobotany, ethnomedicine, and ethnoveterinary estimate that nearly 80% of Ethiopians use some type of medicinal plants and plant remedies. Medicinal plants are regarded as the most important and sometimes the only source of therapeutics in the country. Some 800 plant species are used as sources of medicine to treat about 300 physical and mental disorders. However, because these plant species are not adequately studied, there is a big limitation in their documentation, profiling, and ma...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
In this study, biocomposite films based on polyvinyl alcohol (PVA), gum arabic (GA) and chitosan (CS) incorporated with BPEO and GEO were fabricated by solvent casting method. FTIR, XRD, SEM and DSC were performed with mechanical and antimicrobial properties of PVA/GA/CS films with and without BPEO and GEO. The BPEO-PVA/GA/CS film showed heterogeneous rough surface with cavities containing entrapment of BPEO droplets, whereas, the GEO-PVA/GA/CS film showed heterogeneous rough surface with coarseness due to the incorporation of respective EOs. The BPEO and GEO incorporated PVA/GA/CS films were considerable resistant to brea...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
In this study, a portable analyzer was developed to rapidly and simultaneously detect five types of foodborne bacteria (Staphylococcus aureus, Salmonella, Shigella, enterotoxigenic Escherichia coli, and Pseudomonas aeruginosa) on a centrifugal chip. Integration of the processes, including bacteria lysis, visible loop-mediated amplification (LAMP) and detection, was accomplished within 70 minutes. The analyzer has a compact size (151 × 134 × 110 mm) and two motors positioned on both sides of the centrifugal chip. The upper motor was used to generate a rotating magnetic f...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
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