Pre-analytical variables for the genomic assessment of the cellular and acellular fractions of the liquid biopsy in a cohort of breast cancer patients

Publication date: Available online 22 January 2020Source: The Journal of Molecular DiagnosticsAuthor(s): Stephanie N. Shishido, Lisa Welter, Mariam Rodriguez-Lee, Anand Kolatkar, Liya Xu, Carmen Ruiz, Anna S. Gerdtsson, Sara Restrepo-Vassalli, Anders Carlsson, Joe Larsen, Emily J. Greenspan, E. Shelley Hwang, Kathryn R. Waitman, Jorge Nieva, Kelly Bethel, James Hicks, Peter KuhnAbstractThe liquid biopsy allows assessment of multiple analytes providing temporal information with potential for improving our understanding of cancer evolution and clinical management of patients. While liquid biopsies are intensely investigated for prediction and response monitoring, pre-analytic variables are of primary concern for clinical implementation, including categories of collection methodology and sample storage. Here we utilize an integrated high-definition single cell assay (HD-SCA) workflow for morphometric and genomic analysis of the liquid biopsy to characterize the effects of pre-analytical variation and reproducibility of data from a breast cancer cohort. Following prior work that quantified performance of commonly used blood collection tubes, this study completes the analysis of 4 timepoints to assay (24, 48, 72, 96h), demonstrating precision up to 48h post collection with respect to assay sensitivity, highly reproducible rare cell enumeration, morphometric characterization, and high efficiency and capacity for single cell genomic analysis. For the cell-free analysis, both freezin...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research