Methanol extract of Lonicera caerulea var. emphyllocalyx fruit has anti-motility and anti-biofilm activity against enteropathogenic Escherichia coli.

In this study, we investigated the antibacterial effect of LCE fruit extract (LCEE) against EPEC. The antibacterial activities of LCEE were examined by bacterial growth, time-kill curve, soft-agar motility, electron microscopy, and 96 well-microplate biofilm assays. We also investigated the bacterial mRNA expression of biofilm-associated genes (fliC, csgA, and fimA) by quantitative real-time PCR assays. LCEE was found to suppress the growth, time-kill curve, and spread of EPEC. It also reduced the biofilm formation in a dose-dependent manner. Morphological analysis using transmission and scanning electron microscopy revealed that LCEE diminished the function of flagella resulting in reduced motility and biofilm formation. The mRNA expression of all three biofilm associated genes was downregulated under LCEE treatment. Extracts of the fruit of LCE inhibit the motility and biofilm formation of EPEC as a result of the inhibition of flagella development and function. We propose LCEE as a therapeutic candidate for the effective therapy of EPEC-associated infectious diseases. PMID: 31956232 [PubMed - in process]
Source: Drug Discoveries and Therapeutics - Category: Drugs & Pharmacology Tags: Drug Discov Ther Source Type: research

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Publication date: September 2020Source: Materials Today Communications, Volume 24Author(s): Chanon Talodthaisong, Wissuta Boonta, Saengrawee Thammawithan, Rina Patramanon, Nuntaporn Kamonsutthipaijit, James A. Hutchison, Sirinan KulchatAbstractBiomaterial-based hydrogels incorporating antibacterial agents may provide sustainable solutions to biomedical device failures and the prevention of infections. Herein we report guar gum hydrogels, cross-linked with borax and loaded with silver nanoparticles, that are injectable, exhibit rapid self-healing, and show antibacterial properties towards both gram-positive and gram-negativ...
Source: Materials Today Communications - Category: Materials Science Source Type: research
Abstract Three-dimensional cubic ordered mesoporous carbon with chitosan (Ia3d-CS), which was synthesized via exothermic reaction between liquid potassium and carbon monoxide gas, was coated on the active carbon (AC) electrode as a capacitive deionization (CDI) disinfection electrode. The results showed that Ia3d-CS-2 as CDI electrode exhibited the quick ion diffusion and strong charge transfer performance, due to the three-dimensional pore structure and specific surface area. The electrode of Ia3d-CS-2 displayed a specific capacity of 191.22 F/g at a scan rate of 100 mV·s-1 in 0.5 M NaCl aqu...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
Publication date: Available online 14 February 2020Source: Journal of Molecular StructureAuthor(s): Norasteh Rashidi, Mohammad Jaafar Soltanian Fard, Payam Hayati, Jan Janczak, FatemehYazdianAbstractMononuclear Zinc (II) compound –based nano-structure with [Zn(L)2(CH3COO)2] {as compound 1} formulation was synthesized by reacting ligand 2-Amino-3-Methyl pyridine with Zinc (II) acetate dihydrate and potassium iodide via sonochemical method and characterized by scanning electron microscopy (SEM), X-ray powder diffraction (XRPD), infrared spectroscopy (IR) and elemental analyses. Single crystal X-ray diffraction was used...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Publication date: Available online 12 February 2020Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Hojjat Ghasemi Goojani, Julia Konings, Henk Hakvoort, Sangjin Hong, Robert B. Gennis, Junshi Sakamoto, Holger Lill, Dirk BaldAbstractCytochrome bd, a component of the prokaryotic respiratory chain, is important under physiological stress and during pathogenicity. Electrons from quinol substrates are passed on via heme groups in the CydA subunit and used to reduce molecular oxygen. Close to the quinol binding site, CydA displays a periplasmic hydrophilic loop called Q-loop that is essential for quinol oxi...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - Category: Biochemistry Source Type: research
CONCLUSIONS: We conclude that when two E. coli strains (MTCC723 and MTCC443) and NPs are respectively mixed and kept overnight, the growth of bacteria are inhibited by ZnO-NPs due to changes in cell membrane permeability and intracellular metabolic system under fluorescence microscopy. However, SPR possessed Au-NPs result in enhanced fluorescence of both pathogens. In addition, with the help of Raman microscopy and element analysis, significant changes are observed when Au-NPs are added with the two strains as compared to ZnO-NPs due to protein, lipid and DNA/RNA induced conformational changes. PMID: 32040672 [PubMed ...
Source: Biotechnology Letters - Category: Biotechnology Authors: Tags: Biotechnol Lett Source Type: research
In this study, we explored the effect of three lactic acid bacteria (LAB), i.e. Enterococcus sp CM9, Enterococcus sp CM18 and Enterococcus faecium H3, and their supernatants, on seven biofilm-forming pathogenic strains isolated from human urinary tract or nose infections. By quantitative biofilm production assay, a strong adherence ability of Enterococcus sp CM9 and Enterococcus sp CM18 was revealed while E. faecium H3 resulted to be moderately adherent. Inhibition tests demonstrated an antimicrobial activity of LAB against pathogens.The presence of cell free supernatant (CFS) of CM9 and CM18 strains significantly decrease...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
In conclusion, gas under the diaphragm can be related to non-bowel-related gynaecological pathology, but it vital to rule out sinister causes.
Source: Case Reports in Womens Health - Category: OBGYN Source Type: research
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Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
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Source: Ultrasonics Sonochemistry - Category: Chemistry Authors: Tags: Ultrason Sonochem Source Type: research
Contributors : Eric M Jones ; Nathan B Lubock ; AJ Venkatakrishnan ; Jeffrey Wang ; Alex M Tseng ; Joseph M Paggi ; Naomi R Latorraca ; Daniel Cancilla ; Megan Satyadi ; Jessica E Davis ; M M Babu ; Ron O Dror ; Sriram KosuriSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Escherichia coli ; Homo sapiensIn humans, the 813 G protein-coupled receptors (GPCRs) are responsible for transducing diverse chemical stimuli to alter cell state, and are the largest class of drug targets. Their myriad structural conformations and various modes of signaling make it challenging to understand their struct...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Escherichia coli Homo sapiens Source Type: research
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