Biotin interferences: Have we neglected the impact on serological markers?

Publication date: Available online 21 January 2020Source: Clinica Chimica ActaAuthor(s): Jean-Louis Bayart, Julien Favresse, Anke Stoefs, Mélanie Closset, Tatiana Roy, Catherine Fillée, Hector Rodriguez-Villalobos, Benoît Kabamba-Mukadi, Damien GrusonAbstractBackgroundBiotin has been reported to be a leading cause of interference on several immunoassay platforms using the streptavidin-biotin immobilization system. While biotin interferences have now been well characterized for several assays, only few data are available on their impact on serological markers of infectious viral diseases.MethodsOverall, 10 healthy volunteers (HVs) received a single 100mg dose of biotin to evaluate its effect on hepatitis B serological markers. Blood samples were taken several times before and after biotin intake. In addition, spiking experiments were applied to investigate biotin’s impact on anti-HIV/p24 Ag and anti-HCV antibody levels. Several procedures designed to overcome this interference were evaluated.ResultsBiotin intake resulted in a false-negative anti-HBs immunological status (
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research

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Conclusion: The prevalence of HDV-Ag among asymptomatic treatment naïve chronic hepatitis B patients in Abeokuta was 11% and there was no significant difference in the levels of HBV DNA and HBsAg among those with or without hepatitis D. PMID: 32096684 [PubMed - as supplied by publisher]
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research
CONCLUSIONSWe confirm that seroprotection for vaccine-preventable disease is affected by treatment for pediatric malignancy. A single booster dose of vaccine might be a practical way to restore vaccine immunity in patients after chemotherapy.
Source: Mediterranean Journal of Hematology and Infectious Diseases - Category: Hematology Authors: Source Type: research
Source: Frontiers in Physiology - Category: Physiology Source Type: research
AbstractBackgroundHost genome integration of HBV sequence is considered to be significant in HBV antigen expression and the development of hepatocellular carcinoma (HCC).MethodWe developed a probe-based capture strategy to enrich integrated HBV DNA for deep-sequencing analysis of integration sites in paired patient samples derived from tumor, liver tissue adjacent to tumor, saliva and plasma, as a platform for exploring the correlation, significance and utility of detecting integrations in these sample types.ResultsMost significantly, alpha fetoprotein levels significantly correlated to the amounts of integrations detected...
Source: Hepatology International - Category: Infectious Diseases Source Type: research
ConclusionImmunoprophylaxis may need more consideration for HBsAb-positive patients receiving HBcAb-positive liver grafts. Active vaccination and mono-prophylaxis with NAs could be recommended in HBsAb-negative recipients against DNH. Further studies should examine the higher genetic barrier drugs for preventing DNH, and the association between DNH and HBV DNA-positive liver graft in this patient population.
Source: Hepatology International - Category: Infectious Diseases Source Type: research
Ivanov This short review is focused on enzymatic properties of human ATP-dependent RNA helicase DDX3 and the development of antiviral and anticancer drugs targeting cellular helicases. DDX3 belongs to the DEAD-box proteins, a large family of RNA helicases that participate in all aspects of cellular processes, such as cell cycle progression, apoptosis, innate immune response, viral replication, and tumorigenesis. DDX3 has a variety of functions in the life cycle of different viruses. DDX3 helicase is required to facilitate both the Rev-mediated export of unspliced/partially spliced human immunodeficiency virus (HIV) RNA...
Source: Molecules - Category: Chemistry Authors: Tags: Review Source Type: research
Although immune checkpoint inhibitors (ICIs) have improved survival in many patients with non-small cell lung cancer (NSCLC), [1] our understanding of the safety and efficacy of these therapies in certain populations is limited. Patients with past2 or chronic [1] hepatitis B (HBV) or hepatitis C virus (HCV) infections, for example, have been excluded from most of the pivotal phase 3 immunotherapy clinical trials in lung cancer [1,2], so there is a paucity of data both on the risks of viral reactivation or flare, and on the rates of hepatotoxicity in these patients after treatment with ICIs.
Source: Lung Cancer - Category: Cancer & Oncology Authors: Source Type: research
We described the frequency of disease overlaps; for example, 34.7% of clients with a syphilis record had a gonorrhea record. We quantified known overlaps, finding 1274 clients had gonorrhea, infectious syphilis, and HIV/AIDS records, and potentially emerging overlaps, finding 59 clients had HIV/AIDS, hepatitis C, and iGAS records. IMPLICATIONS: Our novel person-based integrated surveillance dataset represents a platform for ongoing in-depth assessment of disease overlaps such as the relative timing of disease records. It enables a more client-focused approach, is a step towards improved characterization of syndemics i...
Source: Canadian Journal of Public Health - Category: International Medicine & Public Health Authors: Tags: Can J Public Health Source Type: research
Abstract Capsid assembly is a critical step in the hepatitis B virus (HBV) life cycle, mediated by the core protein. Core is a potential target for new antiviral therapies, the capsid assembly modulators (CAMs). JNJ-56136379 (JNJ-6379) is a novel and potent CAM currently in phase II trials. We evaluated the mechanism of action (MOA) and antiviral properties of JNJ-6379 in vitro Size exclusion chromatography and electron microscopy studies demonstrated that JNJ-6379 induced formation of morphologically intact viral capsids devoid of genomic material ('primary' MOA). JNJ-6379 accelerated the rate and extent of HBV c...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
In conclusion, additional NLGSs correlate with HBsAg-negativity despite HBV-reactivation, and hamper HBsAg-antigenicity in vitro, supporting the role of NGSs in immune-escape and the importance of HBV-DNA for a proper diagnosis of HBV-reactivation.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
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