Transcriptional analysis of THP-1 cells infected with < i > Leishmania infantum < /i > indicates no activation of the inflammasome platform

by Mariana Gatto, Patr ícia Aparecida Borim, Ivan Rodrigo Wolf, Taís Fukuta da Cruz, Gustavo Augusto Ferreira Mota, Aline Márcia Marques Braz, Bárbara Casella Amorim, Guilherme Targino Valente, Marjorie de Assis Golim, James Venturini, João Pessoa Araújo Junior, Alessandra Pontillo, Alexandrina Sartori Leishmaniasis is caused by intracellular parasites transmitted to vertebrates by sandfly bites. Clinical manifestations include cutaneous, mucosal or visceral involvement depending upon the host immune response and the parasite species. To assure their survival inside macrophages, these parasites developed a plethora of highly successful strategies to manipulate various immune system pathways. Considering that inflammasome activation is critical for the establishment of a protective immune response in many parasite infections, in this study we determined the transcriptome of THP-1 cells afte r infection withL.infantum, with a particular focus on the inflammasome components. To this end, the human cell line THP-1, previously differentiated into macrophages by PMA treatment, was infected withL.infantum promastigotes. Differentiated THP-1 cells were also stimulated with LPS to be used as a comparative parameter. The gene expression signature was determined 8 hours after by RNA-seq technique. Infected or uninfected THP-1 cells were stimulated with nigericin (NIG) to measure active caspase-1 and TNF- α, IL-6 and IL-1β levels in culture supernatants after 8, 24 and 48 hours....
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research