Enzymatic epoxidation of cyclohexene by peroxidase immobilization on a textile and an adapted reactor design

Publication date: Available online 20 January 2020Source: Enzyme and Microbial TechnologyAuthor(s): D.S. Wunschik, Kim N. Ingenbosch, P. Süss, U. Liebelt, S. Quint, M. Dyllick-Brenzinger, R. Zuhse, U. Menyes, K. Hoffmann-Jacobsen, K. Opwis, J.S. GutmannAbstractA textile-based reaction system for new peroxidase reactions in non-native media was implemented. The epoxidation of cyclohexene by the commercial peroxidase MaxiBright® was realized with the textile-immobilized enzyme in an adapted liquid-liquid two-phase reactor. A commercially available polyester felt was used as low-price carrier and functionalized with polyvinyl amine. The covalent immobilization with glutardialdehyde lead to an enzyme loading of 0.10 genzyme/gtextile. The textile-based peroxidase shows a high activity retention in the presence of organic media. This catalyst is shown to enable the epoxidation of cyclohexene in various solvents as well as under neat conditions. A model reactor was produced by 3D printing which places the textile catalyst at the interphase between the liquid reaction phase and the product extracting solvent.Graphical abstract

Source: Enzyme and Microbial Technology - January 21, 2020 Category: Biotechnology Source Type: research