Nested PCR Amplification Secures DNA Template Quality and Quantity in Real-time mCOP-PCR Screening for SMA.

CONCLUSION: In our study on the real-time mCOP-PCR screening system for SMA, a nested PCR secured the DNA template quality and quantity, leading to unambiguous results of SMA screening.
PMID: 31956257 [PubMed - in process]

Source: Kobe J Med Sci - July 15, 2019 Category: General Medicine Authors: Wijaya YOS, Niba ETE, Rochmah MA, Harahap NIF, Awano H, Takeshima Y, Saito T, Saito K, Takeuchi A, Lai PS, Bouike Y, Nishio H, Shinohara M Tags: Kobe J Med Sci Source Type: research