Treatment with Lycopodium clavatum 200dH Intensifies Kidney and Liver Injury in Mice Infected with Toxoplasma gondii

In this study, physiological, histopathological, and immunological parameters were evaluated. TheL. clavatum 200dH intensified renal damage in mice infected withT. gondii from 7 dpi, causing severe and progressive alterations during this period, such as various degrees of inflammation, edema, atrophy, and tubular cystic dilation, degenerated tubules with intra-cytoplasmic vacuoles and coalescing spots, severe vascular lesions, glomerulonephritis, and peri-glomerular congestion. In the G72 animals, which receivedL. clavatum 200dH, more severe cortex damage was observed (91.66 –96.66%) as compared to the IC group (55–80%) and more renal corpuscle, and renal tubule injury was observed (80 ± 5 to 96.7% ± 2.89 of the total area) during all periods, as compared to the IC group (p  <  0.05). Both groups presented high liver enzyme levels, and the highest values for AST were observable at 60 dpi. We observed significant increases of type I and III collagen, as well as high levels of TGF-β1 in both organs of the treated animals, the main factor involved in fibrosis in areas dam aged by the process.L. clavatum 200dH intensifies kidney and liver alterations in mice infected withT. gondii. Our results reinforce caution when indicating administration schemes and dosages for ultra-diluted drugs.Graphic Abstract
Source: Archivum Immunologiae et Therapiae Experimentalis - Category: Allergy & Immunology Source Type: research