GSE141011 DNA Double Strand Break Repair in E. coli Perturbs Cell Division and Chromosome Dynamics

Contributors : Martin A White ; Elise Darmon ; Manuel A Lopez-Vernaza ; David LeachSeries Type : OtherOrganism : Escherichia coli K-12The effect of a site-specific DNA double-strand break on the abundance of E. coli chromosomal DNA during exponential growth was investigated by marker frequency analysis (MFA).
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Other Escherichia coli K-12 Source Type: research

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The cellular methyl donor S-adenosylmethionine (SAM) and other endo/exogenous agents methylate DNA bases non-enzymatically into products interfering with replication and transcription. An important product is 3-methyladenine (m3A), which in Escherichia coli is removed by m3A-DNA glycosylase I (Tag) and II (AlkA). The tag gene is constitutively expressed, while alkA is induced by sub-lethal concentrations of methylating agents. We previously found that AlkA exhibits activity for the reactive oxygen-induced thymine (T) lesion 5-formyluracil (fU) in vitro. Here, we provide evidence for AlkA involvement in the repair of oxidiz...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, taller body height at the entry to adulthood, supposed to be a marker of early-life environment, is associated with lower risk of dementia diagnosis later in life. The association persisted when adjusted for educational level and intelligence test scores in young adulthood, suggesting that height is not just acting as an indicator of cognitive reserve. A Comparison of Biological Age Measurement Approaches https://www.fightaging.org/archives/2020/02/a-comparison-of-biological-age-measurement-approaches/ Researchers here assess the performance of a range of approaches to measuring biological...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: May 2020Source: Materials Science and Engineering: C, Volume 110Author(s): Ajinkya A. Shitole, Piyush Raut, Prabhanjan Giram, Priyanka Rade, Anand Khandwekar, Baijayantimala Garnaik, Neeti SharmaAbstractFacilitating the process of wound healing and effective treatment of wounds remains a serious challenge in healthcare. Wound dressing materials play a major role in the protection of wounds and in accelerating the natural healing process. In the present study, novel core/shell (c/s) nanofibrous mats of poly(vinyl pyrrolidone)‑iodine (PVPI) and polycaprolactone (PCL) were fabricated using a co-axial elect...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: May 2020Source: Materials Science and Engineering: C, Volume 110Author(s): Xinxin Chen, Xiang Wang, Siyu Wang, Xiuhang Zhang, Jiaao Yu, Ce WangAbstractThere has been a recent increase in research interest regarding the development of wound dressings containing bioactive compounds capable of improving outcomes for complex healing needs. In the present study, we describe the generation of bromelain immobilized eletrospun poly(ε-caprolactone) (PCL) fibers (BrPDA-PCL fibers) using the dopamine-assisted co-deposition strategy. We wanted to combine the structural advantage of electrospun fiber and the a...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Probing the DNA-binding center of the MutL protein from the Escherichia coli mismatch repair system via crosslinking and Förster resonance energy transfer. Biochimie. 2020 Feb 13;: Authors: Monakhova M, Ryazanova A, Kunetky V, Li P, Shilkin E, Kisil O, Rao DN, Oretskaya T, Friedhoff P, Kubareva E Abstract As no crystal structure of full-size MutL bound to DNA has been obtained up to date, in the present work we used crosslinking and Förster resonance energy transfer (FRET) assays for probing the putative DNA-binding center of MutL from Escherichia coli. Several single-cysteine MutL variants ...
Source: Biochimie - Category: Biochemistry Authors: Tags: Biochimie Source Type: research
In conclusion, calcium silicate cement with 1% QTS may be a viable candidate for bone defect repair in view of anti-washout performance, setting time, antibacterial activity, and osteogenic activity shown in this study.
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: 4 February 2020Source: Cell Reports, Volume 30, Issue 5Author(s): Emeline Lawarée, Gytis Jankevicius, Charles Cooper, Ivan Ahel, Stephan Uphoff, Christoph M. TangSummaryADP-ribosylation of proteins is crucial for fundamental cellular processes. Despite increasing examples of DNA ADP-ribosylation, the impact of this modification on DNA metabolism and cell physiology is unknown. Here, we show that the DarTG toxin-antitoxin system from enteropathogenic Escherichia coli (EPEC) catalyzes reversible ADP-ribosylation of single-stranded DNA (ssDNA). The DarT toxin recognizes specific sequence motifs. EPEC ...
Source: Cell Reports - Category: Cytology Source Type: research
Publication date: 15 March 2020Source: Journal of Non-Crystalline Solids, Volume 532Author(s): Honglin Luo, Jian Xiao, Mengxia Peng, Quanchao Zhang, Zhiwei Yang, Hongjuan Si, Yizao WanAbstractA multifunctional bioactive material with osteogenic and antibacterial performance is of crucial importance for the repair of large bone defects. Herein, three-dimensional (3D) mesoporous bioactive glass (MBG) nanofibrous scaffolds doped with copper (Cu) were synthesized via a one-pot template-assisted sol-gel method followed by calcination. The morphology, structure, pore structure, in vitro bioactivity, cell biocompatibility, and an...
Source: Journal of Non Crystalline Solids - Category: Chemistry Source Type: research
This study provides strong evidence that following a healthy lifestyle can substantially extend the years a person lives disease-free." Commentary on Recent Evidence for Cognitive Decline to Precede Amyloid Aggregation in Alzheimer's Disease https://www.fightaging.org/archives/2020/01/commentary-on-recent-evidence-for-cognitive-decline-to-precede-amyloid-aggregation-in-alzheimers-disease/ I can't say that I think the data presented in the research noted here merits quite the degree of the attention that it has been given in the popular science press. It is interesting, but not compelling if its role...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Abstract Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl alcohol) (PVA)/chitosan (CS)/silk sericin (SS)/tetracycline (TCN) porous nanofibers, with diameters varying from 305 to 425 nm, both in vitro and in vivo for potential applications as wound dressings. The fabricated nanofibers possess a considerable capacity to take up water through swelling (~325-650%). Seric...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
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