Cryopreservation of calf testicular tissues with knockout serum replacement.

Cryopreservation of calf testicular tissues with knockout serum replacement.
Cryobiology. 2020 Jan 16;:
Authors: Jiang Y, Zhu WQ, Zhu XC, Cai NN, Yang R, Cai H, Zhang XM
Abstract
To enrich bovine gonocytes from cryopreserved testicular tissues, the cryoprotection effects of the freezing media containing knockout serum replacement (KSR) were examined. Using Minimum essential medium (MEM) + 10% dimethyl sulfoxide (Me2SO) as the basic medium, calf testicular tissues were cryopreserved in media containing 0, 5, 10, 20, 40, 90% KSR and 5% fetal bovine serum (FBS) respectively. Morphologically the seminiferous cords and interstitium were well preserved in all groups. The gonocytes were all glial cell line-derived neurotrophic factor (GDNF) family receptor α-1 (GFRα-1) positive. The recovery rates in all KSR groups were higher than that of the 10% Me2SO group, while comparable to the 5% FBS group. The enriched gonocytes expressed gonocyte marker GFRα-1 typically. Collectively supplementation of 5-10% KSR can achieve comparable cryoprotective effects with using 5% FBS, which is useful in future study due to its defined formulation that is more consistent in quality and stable in supply.
PMID: 31954710 [PubMed - as supplied by publisher]

Source: Cryobiology - January 15, 2020 Category: Biology Authors: Jiang Y, Zhu WQ, Zhu XC, Cai NN, Yang R, Cai H, Zhang XM Tags: Cryobiology Source Type: research