Site-specific profiling of the dental mycobiome reveals strong taxonomic shifts during progression of early childhood caries.

Site-specific profiling of the dental mycobiome reveals strong taxonomic shifts during progression of early childhood caries. Appl Environ Microbiol. 2020 Jan 17;: Authors: O'Connell LM, Santos R, Springer G, Burne RA, Nascimento MM, Richards VP Abstract Dental caries is one of the most common diseases worldwide. Bacteria and fungi are both commensals in the oral cavity; however, most research regarding caries has focused on bacterial impacts. The oral fungal mycobiome associated with caries is not well characterized and its role in disease is unclear. ITS1 amplicon sequencing was used to generate taxonomic profiles from site-specific supragingival plaque samples (n=82) obtained from 33 children with different caries status. Children were either caries-free (CF), caries-active with enamel lesions (CAE), or caries-active with dentin lesions (CA). Plaque samples were collected from caries-free surfaces (PF), and from enamel (PE) and dentin (PD) lesions. Taxonomic profiles representing the different categorizations (CF-PF, CAE-PF, CAE-PE, CA-PF, CA-PE and CA-PD) were utilized to characterize the mycobiome and its change through disease progression. A total of 139 fungal species were identified. Candida albicans was the most abundant species followed by Candida dubliniensis We found that severely progressed plaque communities (CA-PD) were significantly different from healthy plaque communities (CF-PF). A total of 32 taxa were differentia...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research