Inactivating foodborne pathogens in apple juice by combined treatment with fumaric acid and ultraviolet-A light, and mechanisms of their synergistic bactericidal action.

Inactivating foodborne pathogens in apple juice by combined treatment with fumaric acid and ultraviolet-A light, and mechanisms of their synergistic bactericidal action. Food Microbiol. 2020 May;87:103387 Authors: Jeon MJ, Ha JW Abstract We evaluated the bactericidal efficacy of the simultaneous application of ultraviolet-A (UV-A) irradiation and fumaric acid (FA) against Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes in apple juice and as well as investigated the effects of this treatment on product quality. Further, we elucidated the mechanisms underlying their synergistic bactericidal action. Simultaneous UV-A light irradiation and 0.1% FA treatment for 30 min resulted in 6.65-, 6.27-, and 6.49-log CFU/ml reductions in E. coli O157:H7, S. Typhimurium, and L. monocytogenes, respectively, which involved 3.15, 2.21, and 3.43 log CFU reductions, respectively, and these were attributed to the synergistic action of the combined treatments. Mechanistic investigations suggested that the combined UVA-FA treatment resulted in significantly greater bacterial cell membrane damage and intracellular reactive oxygen species (ROS) generation. UVA-FA treatment for 30 min did not cause significant changes to the color, nonenzymatic browning index, pH, and total phenolic content of apple juice. These results suggest that combined UVA-FA treatment can be effectively used to control foodborne pathogens in apple juic...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research

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Publication date: Available online 19 February 2020Source: Materials Chemistry and PhysicsAuthor(s): Natasha Vieira Sievers, Liliane Damaris Pollo, Gertrudes Corção, Nilo Sérgio Medeiros CardozoAbstractThis work reports a new method to obtain polypropylene/titanium dioxide (PP/TiO2) films with antibacterial activity through the in situ synthesis of nanostructured TiO2 directly in the polymer solution. In order to compare the performance of the different types of particles, TiO2 particles were also prepared utilizing the sol-gel method and subsequently incorporated into the polymer to form PP/TiO2 films...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
In this study we designed and synthetized various derivatives of NCR247, including shorter fragments and various chimeric derivatives. The antimicrobial activity of these peptides was tested on the ESKAPE bacteria; Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli as a member of Enterobacteriaceae and in addition Listeria monocytogenes and Salmonella enterica. The 12 amino acid long C-terminal half of NCR247, NCR247C partially retained the antimicrobial activity and preserved the multitarget interactions with partners of NCR247. Nevert...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, unlike traditional alheiras which often contain pathogenic agents, no harmful organisms nor chemical hazards were found in these new products, even though produced by the same companies.
Source: Food Control - Category: Food Science Source Type: research
This study characterized Listeria monocytogenes, Salmonella enterica, and E. coli O157:H7 by collecting rectoanal mucosal swabs and fecal samples from 518 imported beef cattle at Jordan’s major abattoir. A unique 53 L. monocytogenes, 287 S. enterica, and 17 E. coli O157:H7 were isolated from 37, 120 and 9 different animals; respectively. The prevalence of S. enterica, L. monocytogenes and E. coli O157:H7 were 23.2% (95% CI, 19.7-27.0%), 7.1% (95% CI, 5.2-9.7%) and 1.7% (95% CI, 0.9-3.3%); respectively. All L. monocytogenes, all E. coli O157:H7 and 93.0% of S. enterica isolates resisted at least one antimicrobial clas...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
te; T Abstract Bacteria within biofilms are more resistant to antibiotics and chemical agents than planktonic bacteria in suspension. Treatment of biofilm-associated infections inevitably involves high dosages and prolonged courses of antimicrobial agents; therefore, there is a potential risk of the development of antimicrobial resistance (AMR). Due to the high prevalence of AMR and its association with biofilm formation, investigation of more effective anti-biofilm agents is required. From ancient times, herbs and spices have been used to preserve foods, and their antimicrobial, anti-biofilm and anti-quorum sensi...
Source: Current Pharmaceutical Design - Category: Drugs & Pharmacology Authors: Tags: Curr Pharm Des Source Type: research
This study aims to i) evaluate two triple-wash procedures with an H2O2-peroxyacetic-acid mixer (SaniDate-5.0) to improve microbial safety and quality of butternut squashes and ii) determine the feasibility of the triple-wash application in a processing plant. In study I, fresh squashes were dip-inoculated with a mixture of Salmonella and Listeria monocytogenes, followed by two triple-wash steps including water dip-antimicrobial dip-water dip (WAW) or water dip-water dip-antimicrobial dip (WWA). Tested antimicrobials were i) lactic/citric acid blend (LCA; 2.5%); ii) sodium hypochlorite (SH; 100 ppm); and iii) SaniDate-5.0...
Source: Food Control - Category: Food Science Source Type: research
Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific sequences of Salmonella Typhimurium, Vibrio parahemolyticus, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7 from the National Center for Biotechnology Information database. Tiling array probes wer...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study determined the efficacy of UV-C as a decontamination process against some foodborne bacteria in dried whole black peppercorns. Artificially-inoculated Salmonella enterica, Escherichia coli O157:H7, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus were subjected to UV-C with a surface irradiance of 0.43 mW/cm2 and were all found to exhibit a biphasic inactivation pattern with fast log-linear inactivation followed by a tail. Total log reductions (TLR) ranged from 1.92 (S. aureus) to 3.60 log CFU/g (E. coli O157:H7). Increasing the lamp number from 1 to 5 also linearly (R2 = ...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens. J Food Sci. 2020 Jan 30;: Authors: Tao J, Liu W, Ding W, Han R, Shen Q, Xia Y, Zhang Y, Sun W Abstract Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time-consuming and labor-intensive. Mult...
Source: Anal Sci - Category: Chemistry Authors: Tags: J Food Sci Source Type: research
In this study, our aim was to evaluate ClO2 precursors, sodium chlorite and citric acid, under disparate but applicable conditions. First, we modeled production of ClO2 from the powdered precursors with three different masses, sample ID 1, 2, and 3, at room (18 °C) and refrigeration (4 °C) temperatures. Second, we investigated the effects of a water reservoir on ClO2 production. Third, we assessed collection method issues that may impact reported ClO2 values. Finally, we evaluated the precursor's efficacy against three common foodborne pathogens: Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella ...
Source: Innovative Food Science and Emerging Technologies - Category: Food Science Source Type: research
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