Niclosamide suppresses macrophage induced inflammation in endometriosis.

Niclosamide suppresses macrophage induced inflammation in endometriosis. Biol Reprod. 2020 Jan 17;: Authors: Sekulovski N, Whorton AE, Tanaka T, Hirota Y, Shi M, MacLean JA, Mola JRL, Groesch K, Diaz-Sylvester P, Wilson T, Hayashi K Abstract Endometriosis is a common gynecological disease, which causes chronic pelvic pain and infertility in women of reproductive age. Due to limited efficacy of current treatment options, a critical need exists to develop new and effective treatments for endometriosis. Niclosamide is an efficacious and FDA-approved drug for the treatment of helminthosis in humans that has been used for decades. We have reported that niclosamide reduces growth and progression of endometriosis-like lesions via targeting STAT3 and NFĸB signaling in a mouse model of endometriosis. To examine the effects of niclosamide on macrophage-induced inflammation in endometriosis, a total of 29 stage III-IV endometrioma samples were used to isolate human endometriotic stromal cells (hESCs). M1 or M2 macrophages were isolated and differentiated from fresh human peripheral blood samples. Then, hESCs were cultured in conditioned media from macrophages with/without niclosamide. Niclosamide dose-dependently reduced cell viability and the activity of STAT3 and NFκB signaling in hESCs. While macrophage conditioned media stimulated cell viability in hESCs, niclosamide inhibited this stimulation. Macrophage conditioned media stimulated the ...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research