Extracellular hydrolytic enzyme activities of indigenous actinomycetes on pretreated bagasse using choline acetate ionic liquid

In this study hydrolytic activities of cellulase and xylanase which produce extra-cellularly by isolated actinomycetes under the presence of ChOAc were investigated. ChOAc/biomass ratio (g/g) were set up between 0.0-3.0 for pretreatment followed by hydrolysis using cellulase and xylanase that excreted when actinomycetes were grown up in pretreated bagasse successively. Act-7 was used as inoculum in saccharification, and the optimum of ChOAc/biomass ratio was as identified to be 1, which achieved high saccharification with a yield of reducing sugar 74.58%. Since the cellulase activities reduced to around 30–70% while ChOAc/biomass ratio was increased to 2–3, in contrast, xylanase activities remain stable at the range of 58.97–75.19 U/mL. This experiment demonstrates the promising bio-hydrolysis of biomass in green technology to increase the yield of the biomass saccharification process.

Source: Biocatalysis and Agricultural Biotechnology - January 18, 2020 Category: Biotechnology Source Type: research