Transcriptome and proteome analyses reveal selenium mediated amelioration of arsenic toxicity in rice (Oryza sativa L.)

Publication date: Available online 16 January 2020Source: Journal of Hazardous MaterialsAuthor(s): Reshu Chauhan, Surabhi Awasthi, Yuvraj Indoliya, Abhishek Singh Chauhan, Shashank Mishra, Lalit Agrawal, Sudhakar Srivastava, Sanjay Dwivedi, Poonam C. Singh, Shekhar Mallick, Puneet Singh Chauhan, Veena Pande, Debasis Chakrabarty, Rudra Deo TripathiAbstractArsenic (As), a chronic poison and non-threshold carcinogen, is a food chain contaminant in rice, posing yield losses as well as serious health risks. Selenium (Se), a trace element, is a known antagonist of As toxicity. In present study, RNA seq. and proteome profiling, along with morphological analyses were performed to explore molecular cross-talk involved in Se mediated As stress amelioration. The repair of As induced structural deformities involving disintegration of cell wall and membranes were observed upon Se supplementation. The expression of As transporter genes viz., NIP1;1, NIP2;1, ABCG5, NRAMP1, NRAMP5, TIP2;2 as well as sulfate transporters, SULTR3;1 and SULTR3;6, were higher in As + Se compared to As alone exposure, which resulted in reduced As accumulation and toxicity. The higher expression of regulatory elements like AUX/IAA, WRKY and MYB TFs during As + Se exposure was also observed. The up-regulation of GST, PRX and GRX during As + Se exposure confirmed the amelioration of As induced oxidative stress. The abundance of proteins involved in photosynthesis, energy met...
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research

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I’ve often shared the habits and techniques I use to handle stress in my life. But this week I’d like to do something different. I’d like to share other people’s perspectives and quotes on stress. And what to do about that stress so that you can live a healthier, more focused and confident and at the same time more relaxing life. Here are 60 timeless thoughts on stress and how to handle it from the people who came before us (and some from people who are here with us now). 60 Helpful and Inspiring Quotes on Stress “The time to relax is when you don't have time for it.” – Sydne...
Source: Practical Happiness and Awesomeness Advice That Works | The Positivity Blog - Category: Consumer Health News Authors: Tags: Happiness Health Personal Development Relaxation Source Type: blogs
(PHOENIX) — A Phoenix-area man has died and his wife was in critical condition after the couple took chloroquine phosphate, an additive used to clean fish tanks that is also found in an anti-malaria medication that’s been touted by President Donald Trump as a treatment for COVID-19. Banner Health said Monday that the couple in their 60s got sick within half an hour of ingesting the additive. The man couldn’t be resuscitated when he arrived at a hospital, but the woman was able to throw up much of the chemical, Banner said. It’s unclear if the couple took it specifically because of COVID-19, the dise...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized COVID-19 overnight wire Source Type: news
by Barbara Fasulo, Angela Meccariello, Maya Morgan, Carl Borufka, Philippos Aris Papathanos, Nikolai Windbichler Synthetic sex distorters have recently been developed in the malaria mosquito, relying on endonucleases that target the X-chromosome during spermatogenesis. Although inspired by naturally-occurring traits, it has remained unclear how they function and, given their potential for genetic control, ho w portable this strategy is across species. We establishedDrosophila models for two distinct mechanisms for CRISPR/Cas9 sex-ratio distortion —“X-shredding” and “X-poisoning”—and dis...
Source: PLoS Genetics - Category: Genetics & Stem Cells Authors: Source Type: research
lva P Abstract Bivalves were long thought to be "symptomless carriers" of marine microalgal toxins to human seafood consumers. In the past three decades, science has come to recognize that harmful algae and their toxins can be harmful to grazers, including bivalves. Indeed, studies have shown conclusively that some microalgal toxins function as active grazing deterrents. When responding to marine Harmful Algal Bloom (HAB) events, bivalves can reject toxic cells to minimize toxin and bioactive extracellular compound (BEC) exposure, or ingest and digest cells, incorporating nutritional components and toxin...
Source: Developmental and Comparative Immunology - Category: Allergy & Immunology Authors: Tags: Dev Comp Immunol Source Type: research
Topoisomerase II (TOP2) relieves topological stress in DNA by introducing double-strand breaks (DSBs) via a transient, covalently linked TOP2 DNA-protein intermediate, termed TOP2 cleavage complex (TOP2cc). TOP2ccs are normally rapidly reversible, but can be stabilized by TOP2 poisons, such as the chemotherapeutic agent etoposide (ETO). TOP2 poisons have shown significant variability in their therapeutic effectiveness across different cancers for reasons that remain to be determined. One potential explanation for the differential cellular response to these drugs is in the manner by which cells process TOP2ccs. Cells are th...
Source: eLife - Category: Biomedical Science Tags: Genetics and Genomics Source Type: research
Publication date: Available online 22 January 2020Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Donna Goodenow, Faith Emmanuel, Chase Berman, Mark Sahyouni, Christine RichardsonAbstractBioflavonoids have a similar chemical structure to etoposide, the well-characterized topoisomerase II (Top2) poison, and evidence shows that they also induce DNA double-strand breaks (DSBs) and promote genome rearrangements. The purpose of this study was to determine the kinetics of bioflavonoid-induced DSB appearance and repair, and their dependence on Top2. Cells were exposed to bioflavonoids individu...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research
Abstract Topoisomerase poisons are known to stabilize covalent enzyme-DNA intermediates forming covalent cleavage complexes, which are highly cytotoxic especially for dividing cells and thus, make topoisomerases targets for cancer therapy. Topoisomerases have been extensively studied in mammalian model systems, whereas in other vertebrate models including zebrafish, they still remain less characterized. Here we show similarities in the genotoxic effects of zebrafish and mammalian systems towards topoisomerase I (Top1) poisons and PARP inhibitor - olaparib. On the other hand we observed that topoisomerase II (Top2)...
Source: DNA Repair - Category: Genetics & Stem Cells Authors: Tags: DNA Repair (Amst) Source Type: research
Abstract The compaction of DNA and the continuous action of DNA transactions, including transcription and DNA replication, create complex DNA topologies that require Type IIA Topoisomerases, which resolve DNA topological strain and control genome dynamics. The human TOP2 enzymes catalyze their reactions via formation of a reversible covalent enzyme DNA-protein crosslink, the TOP2 cleavage complex (TOP2cc). Spurious interactions of TOP2 with DNA damage, environmental toxicants and chemotherapeutic "poisons" perturbs the TOP2 reaction cycle, leading to an accumulation of DNA-protein crosslinks, and ultimat...
Source: Cellular and Molecular Life Sciences : CMLS - Category: Cytology Authors: Tags: Cell Mol Life Sci Source Type: research
In conclusion, the occupational tasks influenced personal airborne lead exposure levels, which in turn was an important predictor of blood lead levels. The study recommends lead exposure assessments, medical screening and intervention measures to minimize the risk and consequences of occu pational exposures to lead among the study population.
Source: Environmental Geochemistry and Health - Category: Environmental Health Source Type: research
Abstract Vibrio parahemolyticus is a halophilic bacterium which causes widespread seafood poisoning pathogenicity. Although the incidence of disease caused by V. parahemolyticus was stepwise increased, the pathogenic mechanism remained unclear. Herein, the difference of V. parahemolyticus's metabonomic which on blood agar and seawater beef extract peptone medium was detected via nuclear magnetic resonance and 55 metabolites were identified. Among them, 40 kinds of metabolites were upregulated in blood agar group, and 12 kinds were downregulated. Nine pathways were verified by enrichment analysis which were predict...
Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
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