Diagnostic parameters and reliability of four rapid immunochromatographic tests for dengue 4

Publication date: Available online 16 January 2020Source: The Brazilian Journal of Infectious DiseasesAuthor(s): Veronica Elizabeth Mata, Sonia Regina Lambert Passos, Maria Angelica Borges dos Santos, Sibelle Nogueira Buonora, Carlos Augusto Ferreira de Andrade, Monique da Rocha Queiroz Lima, Betina Moreira Costa, Yara Hahr Marques HökerbergAbstractBackgroundAlthough performance of rapid immunochromatographic tests (RITs) for dengue virus (DENV) serotypes 1, 2 and 3 is relatively settled, evidence on accuracy of RITs for DENV-4 are based on studies with small sample sizes and with discrepant results.ObjectivesTo assess accuracy and inter-observer agreement of RITs targeting dengue nonstructural protein-1 (NS1) antigen - Dengue NS1-Bioeasy™, Dengue NS1 Ag Strip-Bio-Rad™, IVB Dengue Ag NS1-Orangelife™ and Dengue NS1-K130-Bioclin™ in DENV-4 samples.MethodsStudy sample (n = 324) included adults presenting at an emergency unit in Rio de Janeiro, Brazil, with fever of ≤72 h and two or more dengue symptoms. A serum sample from each patient was tested by each RIT and considered a positivive reverse-transcription polymerase chain reaction as the gold-standard for dengue diagnosis. The diagnostic parameters analyzed for each RIT were sensitivity, specificity, positive and negative predictive values, and likelihood ratios. Each RIT was read by homogeneous (two junior nurses) or heterogeneous (one junior nurse and one senior biologist) pairs. Agreeme...
Source: The Brazilian Journal of Infectious Diseases - Category: Infectious Diseases Source Type: research

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