Mechanistic evaluation of gastro-protective effects of KangFuXinYe on indomethacin-induced gastric damage in rats

Publication date: January 2020Source: Chinese Journal of Natural Medicines, Volume 18, Issue 1Author(s): Qi-Juan LI, Zhan-Guo WANG, Yu XIE, Qiao LIU, Hui-Ling HU, Yong-Xiang GAOAbstractKangFuXinYe (KFX), the ethanol extract of the dried whole body of Periplaneta americana, is a well-known important Chinese medicine preparation that has been used to treat digestive diseases such as gastric ulcers for many years in China. However, its therapeutic effect and mechanism are not yet well understood. Thus, the aim of this study was to investigate the gastro-protective effects of KangFuXinYe (KFX) in indomethacin-induced gastric damage. Rats were randomly divided into six groups as follows: control, treated with indomethacin (35 mg·kg−1), different dosages of KFX (2.57, 5.14 and 10.28 mL·kg−1, respectively) plus indomethacin, and sucralfate (1.71 mL·kg−1) plus indomethacin. After treatment, rat serum, stomach and gastric homogenates were collected for biochemical tests and examination of histopathology firstly. Rat serum was further used for metabolomics analysis to research possible mechanisms. Our results showed that KFX treatment alleviated indomethacin-induced histopathologic damage in rat gastric mucosa. Meanwhile, its treatment significantly increased cyclooxygenase-1 (COX-1), prostaglandin E2 (PGE2) and epidermal growth factor (EGF) levels in rat serum and gastric mucosa. Moreover, KFX decreased cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) levels. Nine metabolite...
Source: Chinese Journal of Natural Medicines - Category: Complementary Medicine Source Type: research