Cancers, Vol. 12, Pages 232: Ex-Vivo Treatment of Tumor Tissue Slices as a Predictive Preclinical Method to Evaluate Targeted Therapies for Patients with Renal Carcinoma
Cancers, Vol. 12, Pages 232: Ex-Vivo Treatment of Tumor Tissue Slices as a Predictive Preclinical Method to Evaluate Targeted Therapies for Patients with Renal Carcinoma
Cancers doi: 10.3390/cancers12010232
Authors:
Caroline Roelants
Catherine Pillet
Quentin Franquet
Clément Sarrazin
Nicolas Peilleron
Sofia Giacosa
Laurent Guyon
Amina Fontanell
Gaëlle Fiard
Jean-Alexandre Long
Jean-Luc Descotes
Claude Cochet
Odile Filhol
Clear cell renal cell carcinoma (ccRCC) is the third type of urologic cancer. At time of diagnosis, 30% of cases are metastatic with no effect of chemotherapy or radiotherapy. Current targeted therapies lead to a high rate of relapse and resistance after a short-term response. Thus, a major hurdle in the development and use of new treatments for ccRCC is the lack of good pre-clinical models that can accurately predict the efficacy of new drugs and allow the stratification of patients into the correct treatment regime. Here, we describe different 3D cultures models of ccRCC, emphasizing the feasibility and the advantage of ex-vivo treatment of fresh, surgically resected human tumor slice cultures of ccRCC as a robust preclinical model for identifying patient response to specific therapeutics. Moreover, this model based on precision-cut tissue slices enables histopathology measurements as tumor architecture is retained, including the spatial relationship between the tumor and tumor-infiltrating lymphocytes and the stromal compo...
Source: Cancers - Category: Cancer & Oncology Authors: Caroline Roelants Catherine Pillet Quentin Franquet Cl ément Sarrazin Nicolas Peilleron Sofia Giacosa Laurent Guyon Amina Fontanell Ga ëlle Fiard Jean-Alexandre Long Jean-Luc Descotes Claude Cochet Odile Filhol Tags: Article Source Type: research
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