Generation of an induced pluripotent stem cell line (SDQLCHi009-A) from a patient with 47,XXY and Ornithine Transcarbamylase Deficiency carrying a hemizygote mutation in OTC

Publication date: Available online 17 January 2020Source: Stem Cell ResearchAuthor(s): Xiaomeng Yang, Beibei Yan, Haiyan Zhang, Yanyan Ma, Qi Zhou, Yue Li, Jingyun Guan, Dong Wang, Yi Liu, Zhongtao GaiAbstractAn induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells of a 3-day-old boy with 47,XXY and Ornithine Transcarbamylase Deficiency carrying hemizygote mutation (c.663+2T>G (sliping)) in OTC. The iPSCs had original 47,XXY, and mutation in OTC, expressing pluripotency markers and bearing differentiation potential in vitro.
Source: Stem Cell Research - Category: Stem Cells Source Type: research

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Conclusion Consequently, characterization of epidural ASCs, will pave the way for new studies investigating their use in dural and also central nervous system healing and recovery after an injury. PMID: 32222128 [PubMed - as supplied by publisher]
Source: Turkish Journal of Medical Sciences - Category: General Medicine Tags: Turk J Med Sci Source Type: research
Extracorporeal membrane oxygenation (ECMO) is a well-known therapy for refractory cardiac and respiratory failure. Stem cell therapy has been investigated as an adjunctive treatment for use during ECMO, but little is known about the viability of stem cells during ECMO support. We evaluated the viability and activity of mesenchymal stem cells (MSCs) in ex vivo circulation (EVC) conditions. The experimental groups were divided into two subgroups: EVC with oxygenator (OXY group) and EVC without oxygenator (Non-OXY group). Mesenchymal stem cells (1.0 × 107) were injected into the EVC system. Cell counting, a lactate dehy...
Source: ASAIO Journal - Category: Medical Devices Tags: Clinical Critical Care Source Type: research
Publication date: Available online 29 March 2020Source: Stem Cell ResearchAuthor(s): Hannah Döpper, Marius Horstmann, Julia Menges, Morgane Bozet, Deniz Kanber, Laura Steenpass
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 29 March 2020Source: Stem Cell ResearchAuthor(s): Muwan Chen, Muyesier Maimaitili, Susanne Hvolbøl Buchholdt, Uffe Birk Jensen, Fabia Febbraro, Mark Denham
Source: Stem Cell Research - Category: Stem Cells Source Type: research
STEM CELLS, EarlyView.
Source: Stem Cells - Category: Stem Cells Tags: Corrigendum Source Type: research
The study demonstrates that the essential role of RhoA –YAP–β‐catenin signaling axis on inner ear progenitor cells expansion. The response of inner ear progenitor cells to mechanical cues from their microenvironment via RhoA‐mediated actin cytoskeleton contraction, which lead to nuclear Yes‐associated protein (YAP) accumulation. YAP as a medi ator of mechanotransduction signaling to promote the expansion of inner ear progenitor cells through regulating β‐catenin activity. AbstractCellular mechanotransduction plays an essential role in the development and differentiation of many cell types, but...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research
Schematics of the experimental protocol used to demonstrate the selective anti ‐tumor effect of the OSKM factors in vivo. Briefly, HSPCs from reprogrammable mice were isolated, infected in culture for 2 days with a retrovirus containing MLL‐AF9‐GFP and transplanted into sublethally irradiated mice. One week later the percentage of GFP+ cells in the bone marrow and bloo d was determined and animals treated with doxycycline (doxy) for 1 week to activate OSKM in the donor cells or left untreated (no doxy). The mice were then monitored for several months for the onset of leukemia and death. While untreated animals...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Commentary Source Type: research
In this study, we show that niche intrinsic expression of the CXCR4 chemokine receptor critically regulates HSPC maintenance during steady state, and promotes early hematopoietic regeneration after myeloablative irradiation. At steady state, chimeric mice with wild ‐type (WT) HSPC and marrow stroma that lack CXCR4 show decreased HSPC quiescence, and their repopulation capacity was markedly reduced. Mesenchymal stromal cells (MSC) were significantly reduced in the BM of CXCR4 deficient mice, which was accompanied by decreased levels of the HSPC supporting fac tors stromal cell‐derived factor‐1 (SDF‐1) and stem cell ...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research
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Source: Stem Cells - Category: Stem Cells Authors: Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research
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Source: Protein and Cell - Category: Cytology Tags: Protein Cell Source Type: research
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