Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection

by C éline Couturier, Atsuhiko Wada, Karen Louis, Maxime Mistretta, Benoit Beitz, Moriba Povogui, Maryline Ripaux, Charlotte Mignon, Bettina Werle, Adrien Lugari, Delphine Pannetier, Sabine Godard, Anne Bocquin, Stéphane Mely, Ismaël Béavogui, Jean Hébélamou, David Leuenberger, Philippe Leissner, T akeshi Yamamoto, Patrick Lécine, Christophe Védrine, Julie Chaix Hemorrhagic fever outbreaks are difficult to diagnose and control in part because of a lack of low-cost and easily accessible diagnostic structures in countries where etiologic agents are present. Furthermore, initial clinical symptoms are common and shared with other endemic diseases such as mala ria or typhoid fever. Current molecular diagnostic methods such as polymerase chain reaction require trained personnel and laboratory infrastructure, hindering diagnostics at the point of need, particularly in outbreak settings. Therefore, rapid diagnostic tests such as lateral flow can be broadly d eployed and are typically well-suited to rapidly diagnose hemorrhagic fever viruses, such as Ebola virus. Early detection and control of Ebola outbreaks require simple, easy-to-use assays that can detect very low amount of virus in blood. Here, we developed and characterized an immunoassay test base d on immunochromatography coupled to silver amplification technology to detect the secreted glycoprotein of EBOV. The glycoprotein is among the first viral proteins to be detected...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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