Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection
by C éline Couturier, Atsuhiko Wada, Karen Louis, Maxime Mistretta, Benoit Beitz, Moriba Povogui, Maryline Ripaux, Charlotte Mignon, Bettina Werle, Adrien Lugari, Delphine Pannetier, Sabine Godard, Anne Bocquin, Stéphane Mely, Ismaël Béavogui, Jean Hébélamou, David Leuenberger, Philippe Leissner, T akeshi Yamamoto, Patrick Lécine, Christophe Védrine, Julie Chaix Hemorrhagic fever outbreaks are difficult to diagnose and control in part because of a lack of low-cost and easily accessible diagnostic structures in countries where etiologic agents are present. Furthermore, initial clinical symptoms are common and shared with other endemic diseases such as mala ria or typhoid fever. Current molecular diagnostic methods such as polymerase chain reaction require trained personnel and laboratory infrastructure, hindering diagnostics at the point of need, particularly in outbreak settings. Therefore, rapid diagnostic tests such as lateral flow can be broadly d eployed and are typically well-suited to rapidly diagnose hemorrhagic fever viruses, such as Ebola virus. Early detection and control of Ebola outbreaks require simple, easy-to-use assays that can detect very low amount of virus in blood. Here, we developed and characterized an immunoassay test base d on immunochromatography coupled to silver amplification technology to detect the secreted glycoprotein of EBOV. The glycoprotein is among the first viral proteins to be detected...
Chinese leaders signaled optimism about controlling the outbreak, as some businesses began to reopen. But restrictions on mobility remained tight.
Japan's financial watchdog has begun conducting an emergency survey on domestic financial institutions with business operations in China to gauge how the coronavirus outbreak could affect credit costs, government sources said on Wednesday.
Mongolia will keep all its schools shut until March 30 in order to prevent the coronavirus from spreading in the country, the government said on Wednesday.
South Korea reported 15 new cases of the coronavirus on Wednesday, including 10 people involved in a surprise outbreak traced to several church services in the central city of Daegu.
There are no indications that there are cases of the new coronavirus in North Korea, a World Health Organization (WHO) official said, despite South Korean media reports suggesting the outbreak had spread to the isolated country.
As the virus-stricken Diamond Princess cruise ship is evacuated, Japan faces deepening criticism over its response to the outbreak, tarnishing what was supposed to be a triumphant Olympic year for Prime Minister Shinzo Abe.
A viral outbreak that began in China has infected more than 75,000 people globally
The outbreak has exposed the powerlessness of private charities, civic groups and others who could help the effort but whom the Communist Party considers rivals.
Mostly innocuous and fairly unknown until a few weeks ago, the Zika virus is suddenly dominating the news. Under scrutiny is the virus's putative link with a congenital birth defect called microcephaly, which causes babies to be born with abnormally small heads and undeveloped brains. Two recent publications [1,2] have documented finding the genome of the Zika virus in the amniotic fluid and brains of fetuses affected by microcephaly from three different mothers. These numbers are still too small to constitute a proof, and in fact, alternative theories are already cropping up: an organization of Argentinean doctors has pu...
Conclusion How many vaccinations will be considered to be a sensible number? If all of the vaccinations currently under development are deemed a success, how many of them will be added to the schedule? As there is little research to determine which ingredients are in the vaccinations listed as “under development” by the CDC, many parents are concerned about their toxicity and how best to protect their children. I will leave you with the wise words of Robert F, Kennedy Jr: “Vaccine industry money has neutralized virtually all of the checks and balances that once stood between a rapacious pharmaceutical ind...