Viruses, Vol. 12, Pages 115: Population- and Variant-Based Genome Analyses of Viruses from Vaccine-Derived Rabies Cases Demonstrate Product Specific Clusters and Unique Patterns
Viruses, Vol. 12, Pages 115: Population- and Variant-Based Genome Analyses of Viruses from Vaccine-Derived Rabies Cases Demonstrate Product Specific Clusters and Unique Patterns
Viruses doi: 10.3390/v12010115
Authors:
Sten Calvelage
Marcin Smreczak
Anna Orłowska
Conrad Martin Freuling
Thomas Müller
Christine Fehlner-Gardiner
Susan Nadin-Davis
Dirk Höper
Paweł Trębas
Rabies in wildlife has been successfully controlled in parts of Europe and North America using oral rabies vaccination, i.e., the distribution of baits containing live-attenuated virus strains. Occasionally, these vaccines caused vaccine virus-induced rabies cases. To elucidate the mechanisms of genetic selection and the effect of viral populations on these rabies cases, a next generation sequencing approach as well as comprehensive data analyses of the genetic diversity of Street Alabama Dufferin (SAD) and ERA vaccine virus strains and vaccine-induced rabies cases from Canada and several European countries were conducted. As a result, twelve newly generated sets of sequencing data from Canada and Poland were added to a pool of previously investigated samples. While the population-based analysis showed a segregation of viruses of ERA vaccine-induced rabies cases from those of SAD Bern original (SAD Bernorig)-derived rabies cases, the in-depth variant analysis revealed three distinct combinations of selected variants for the ERA vaccine-induced cases, suggesting the presence of multiple...
Source: Viruses - Category: Virology Authors: Sten Calvelage Marcin Smreczak Anna Or łowska Conrad Martin Freuling Thomas M üller Christine Fehlner-Gardiner Susan Nadin-Davis Dirk H öper Pawe ł Trębas Tags: Article Source Type: research