Colorimetric-based detection of Ureaplasma urealyticum using gold nanoparticles

Ureaplasma urealyticum (uu) is one of the most common agents of urogenital infections and is associated with complications such as infertility, spontaneous abortion and other sexually transmitted diseases. Here, a DNA sensor based on oligonucleotide target-specific gold nanoparticles (AuNPs) was developed, in which the dispersed and aggregated states of oligonucleotide-functionalised AuNPs were optimised for the colorimetric detection of a polymerase chain reaction (PCR) amplicon of U. urealyticum DNA. A non-cross-linking approach utilising a single Au-nanoprobe specific of the urease gene was utilised and the effect of a PCR product concentration gradient evaluated. Results from both visual and spectral analyses showed that target–Au-nanoprobe hybrids were stable against aggregation after adding the inducer. Furthermore, when a non-target PCR product was used, the peak position shifted and salt-induced aggregation occurred. The assay's limit of detection of the assay was 10 ng with a dynamic range of 10–60 ng. This procedure provides a rapid, facile and low-cost detection format, compared to methods currently used for the identification of U. urealyticum.
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research

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ConclusionCombining different computational approaches including some that were not developed to investigate missense variants help to predict the possible impact of the amino acid substitutions. Yet, when the modified residues are involved in a salt ‐bridge, the tools tend to fail, even when the analysis is performed in 3D. Thus, interactive structural analysis with molecular graphics packages such as Chimera or PyMol or others are still needed to clarify automatic prediction.
Source: Molecular Genetics & Genomic Medicine - Category: Genetics & Stem Cells Authors: Tags: ORIGINAL ARTICLE Source Type: research
Discussion and conclusion.TheFSHB c. ‐211G >  T polymorphism does not affect SC numbers or SCWL, thereby in principle maintaining the spermatogenic potential. The previously observed clinical phenotype for theFSHB genotype might therefore be caused by a hypo ‐stimulated spermatogenesis and not due to a decreased SC number.
Source: Andrology - Category: Urology & Nephrology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Conditions:   Hypertension;   Salt; Excess Interventions:   Device: Salt-meter;   Behavioral: Education Sponsor:   Mahidol University Active, not recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Infertility, Male Intervention:   Sponsor:   Assiut University Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conclusion The increase in antimicrobial resistance towards drugs used to treat gonorrhoea is reaching a critical stage, especially given how common the infection is worldwide, with an estimated 78 million new cases in 2012. This study raises concerns around an important topic while also proposing strategies to help address the slow pace of research and development of new drugs. The prevention of gonorrhoea is equally, if not more, important. The most effective way to prevent gonorrhoea is to always use a condom during sex, including anal and oral sex. Read more advice about sexually transmitted infections and how to prev...
Source: NHS News Feed - Category: Consumer Health News Tags: Medication QA articles Source Type: news
Andrea Syrtash was first hospitalized at the age of 14 for painful and heavy menstrual cycles due to endometriosis. She had no idea her condition would affect her fertility ― and even if she had known, she may not have thought to address it without guidance from her doctors. After six years of trying to conceive, Syrtash, who’s now in her 40s and works as a relationship and dating expert, recently founded pregnantish, a website for singles, couples and LGBTQ people who are trying to conceive.  “When you’re a teenager, it’s not on your mind,” she said. Had she known, “I migh...
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
Conclusions: Our findings confirm the association between previous exposure to C. trachomatis and ectopic pregnancy. We found no association between C. trachomatis seropositivity and miscarriage or preterm birth.
Source: Sexually Transmitted Diseases - Category: Sexual Medicine Tags: Original Study Source Type: research
Authors: Salfa MC, Suligoi B, Italian STI Laboratory-based Surveillance Working Group Abstract Bacterial and protozoal sexually transmitted infections (STIs), such as Chlamydia trachomatis, Trichomonas vaginalis and Neisseria gonorrhoeae, may cause acute symptoms, chronic infections and severe long-term complications. The complications of these infections in women include pelvic inflammatory disease, chronic pelvic pain, tubal infertility, ectopic pregnancy, and infertility. Moreover, infection during pregnancy is associated with premature rupture of the membranes, low birth weight and miscarriage.In Italy, Chlamyd...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
Mycoplasma genitalium has been causally linked with nongonococcal urethritis in men and cervicitis, pelvic inflammatory disease, preterm birth, spontaneous abortion, and infertility in women, yet treatment has proven challenging. To inform treatment recommendations, we reviewed English-language studies describing antimicrobial susceptibility, resistance-associated mutations, and clinical efficacy of antibiotic therapy, identified via a systematic search of PubMed supplemented by expert referral. Minimum inhibitory concentrations (MICs) from some contemporary isolates exhibited high-level susceptibility to most macrolides a...
Source: Clinical Infectious Diseases - Category: Infectious Diseases Authors: Tags: EVIDENCE PAPERS FOR THE CDC SEXUALLY TRANSMITTED DISEASES TREATMENT GUIDELINES Source Type: research
The world became a better place recently when world leaders adopted the Sustainable Development Goals (SDGs), a milestone United Nations statement that will shape world policy for the next 15 years to reduce poverty and set us on a more equitable and sustainable trajectory. Sewn centrally into this extensive policy fabric is improving the status of women through expanding reproductive health and rights, achievements that clearly impact equity, educational attainment, and the well-being of women and families. Women’s ability to determine the timing and spacing of children and to avoid sexually transmitted infections (...
Source: Health Affairs Blog - Category: Health Management Authors: Tags: Costs and Spending Drugs and Medical Technology Equity and Disparities Featured Global Health Long-term Services and Supports Public Health Quality family planning HIV/AIDS Reproductive Health sexually transmitted infections sustai Source Type: blogs
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