Interleukin-2-mediated stat-5 expression levels in CD8+CD25+ regulatory T cells: the relevance for asthma.

Interleukin-2-mediated stat-5 expression levels in CD8+CD25+ regulatory T cells: the relevance for asthma. J Biol Regul Homeost Agents. 2019 Nov-Dec;33(6):1747-1754 Authors: Eusebio M, Kraszula L, Kupczyk M, Kuna P, Pietruczuk M Abstract Preliminary studies demonstrated the potential role of CD8+CD25+ T regulatory cells (Tregs) in asthma. However, published data with regard to the relevance of signaling pathways that govern Tregs homeostasis are limited and conflicting. The first aim of this study was to characterize the phosphorylation of STAT-5 in CD8+CD25+ Tregs. The second aim was to investigate the ability of CD8+CD25+ Tregs from patients and controls to respond to interleukin (IL)-2 treatment in vitro. Twenty-five healthy subjects (NC) and 50 patients with either severe (SA) or mild-moderate (MA) asthma were enrolled in the study. STAT-5 phosphorylation was detected in purified CD8+CD25+ Tregs from healthy and asthma subjects, indicating that STAT-5 has a role in their pathobiology. At baseline, asthmas had either significantly lower [(SA=4.5±5% vs NC=26±25%, P < 0.001) and (MA=10±7.5% vs NC=26±25%, P < 0.001)] or higher [(SA=54±58.5% vs 26±25%, P < 0.01) and (MA=71±74.5% vs 26±25%, P < 0.01) proportion of Tregs expressing pSTAT-5 than controls. In contrast to healthy subjects, CD8+CD25+ Tregs from asthma subjects had either increased (pSTAT-5high) or decreased (pSTAT-5low) phosphorylated STAT-5 levels withi...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research