Cancers, Vol. 12, Pages 219: Cell Electropermeabilisation Enhancement by Non-Thermal-Plasma-Treated PBS

Cancers, Vol. 12, Pages 219: Cell Electropermeabilisation Enhancement by Non-Thermal-Plasma-Treated PBS Cancers doi: 10.3390/cancers12010219 Authors: Thai-Hoa Chung Augusto Stancampiano Kyriakos Sklias Kristaq Gazeli Franck M. André Sébastien Dozias Claire Douat Jean-Michel Pouvesle João Santos Sousa Éric Robert Lluis M. Mir The effectiveness of electrochemotherapy (ECT) in local eradication of tumours in human and veterinary medicine has been proven. ECT consists of increasing the uptake of cytotoxic drugs by means of pulsed electric fields (PEFs) that transiently permeabilise the cell membrane. Still, this tumour treatment includes some drawbacks that are linked to the characteristics of the intense electric pulses (EPs) used. Meanwhile, the emerging field of cancer therapies that are based on the application of non-thermal plasmas (NTP) has recently garnered interest because of their potentialities as rich sources of reactive species. In this work, we investigated the potential capabilities of the combined application of indirect NTP treatment and microsecond PEFs (µsPEFs) to outperform in vitro cell electropermeabilisation, the basis of ECT. Thus, phosphate-buffered saline (PBS) was plasma-treated (pPBS) and used afterwards to explore the effects of its combination with µsPEFs. Analysis of two different cell lines (DC-3F Chinese hamster lung fibroblasts and malignant B16-F10 murine melanoma cells), by flow cytome...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research