Non-covalent functionalization of graphene oxide using self-assembly of silver-triphenylphosphine for bactericidal formulations

Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad BelbahriAbstractGO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction analysis (XRD), Raman spectroscopy, Thermo-gravimetric analysis (TGA), X-ray fluorescence (XRF), Zeta potential analysis, Scanning electron microscopy (SEM), and Atomic force microscopy (AFM) techniques were used to reveal that AgNPs have covered the surface of GO sheets through non covalent and permanent bonding, altering new structural and electronic properties, leading to the appearance of the oxidative stress phenomenon, which considered as a key step of the antibacterial mechanism of this kind of hybrids, causing death of both Gram-positive (Bacillus subtilis, Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus - MRSA, and Staphylococcus aureus) and Gram-negative (Escherichia coli, Serratia marcescens, Shigella sp, Salmonella sp, Serratia microorganisms liquefaciens, Proteus sp, Enterobacter cloacae and Pseudomonas aeruginosa).Graphical abst...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research

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This study compares the effect of Helicobacter (H.) pylori and Lactobacillus (L.) acidophilus on the cytokine profile of PBMCs of 5 men with abdominal aortic aneurysm (AAA) and 5 men with normal/insignificant angiography, CT-Scan and ultrasonography results in the single-culture and in the co-culture with HUVECs. IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17A, IL-17 F, IL-21, IL-22, IFN-γ and TNF-α were measured in culture supernatants using a commercial fluorescent-labeled-bead assay.In general, CagA+ H. pylori-extract induced higher production of IFN-γ, IL-13 and IL-21 by PBMCs. Treatment of ...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
This study characterized Listeria monocytogenes, Salmonella enterica, and E. coli O157:H7 by collecting rectoanal mucosal swabs and fecal samples from 518 imported beef cattle at Jordan’s major abattoir. A unique 53 L. monocytogenes, 287 S. enterica, and 17 E. coli O157:H7 were isolated from 37, 120 and 9 different animals; respectively. The prevalence of S. enterica, L. monocytogenes and E. coli O157:H7 were 23.2% (95% CI, 19.7-27.0%), 7.1% (95% CI, 5.2-9.7%) and 1.7% (95% CI, 0.9-3.3%); respectively. All L. monocytogenes, all E. coli O157:H7 and 93.0% of S. enterica isolates resisted at least one antimicrobial clas...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
Publication date: Available online 19 February 2020Source: European Annals of Otorhinolaryngology, Head and Neck DiseasesAuthor(s): M. Risoud, M. Tardivel, P.-E. Lemesre, N.-X. Bonne, C. VincentAbstractImmunofluorescence on cleared intact cochlea allows detailed analysis of the cochlear ultrastructure, while avoiding the problems of dissection and serial sections. Protocols have been developed for mice and Mongolian gerbils. This technical note proposes a detailed and optimised immunofluorescence protocol in the Mongolian gerbil comprising significant quantitative and qualitative improvements. This protocol sequentially co...
Source: European Annals of Otorhinolaryngology, Head and Neck Diseases - Category: ENT & OMF Source Type: research
CONCLUSION: The use of RGP prior to ureteral stent placement for an obstructing ureteral stone with concomitant UTI was not associated with unfavorable clinical outcomes in our retrospective series. While these findings support the safety of RGP in this setting, prospective trials are warranted. PMID: 32065871 [PubMed - in process]
Source: Canadian Journal of Urology - Category: Urology & Nephrology Tags: Can J Urol Source Type: research
Authors: Mann M, Calio BP, Mark JR, Chelluri R, Hufnagel E, Reese A, Lallas CD, Trabulsi EJ, Chandrasekar T, Shenot PJ, Halpern E, Gomella LG Abstract INTRODUCTION: To assess whether standard American Urological Association (AUA) and other recommendations for prostate biopsy prophylaxis provide sufficient coverage of common urinary organisms responsible for post biopsy infections by comparing local antibiograms in Philadelphia-area hospitals. MATERIALS AND METHODS: De-identified culture results derived from antibiograms were collected from six academic and community hospitals in the Philadelphia region. Analysi...
Source: Canadian Journal of Urology - Category: Urology & Nephrology Tags: Can J Urol Source Type: research
ConclusionThere was an increase in the prevalence of neuroischemic DFU. The Enterobacteriaceae family replaced SA as the most prevalent pathogen in DFI, with an increase in the isolation of gram-negative microorganisms and mixed polymicrobial cultures. Chronic neuroischemic infected ulcers usually present distinct bacterial isolates; knowledge about the most common agents is warranted in order to better select empiric antibiotic therapy.
Source: Diabetes and Metabolic Syndrome: Clinical Research and Reviews - Category: Endocrinology Source Type: research
CONCLUSION: Long-term survival following EPS managed conservatively with nutritional support is feasible, with the majority no longer requiring nutritional support and having a quality of life similar to other patients with ESKD. PMID: 32067553 [PubMed - as supplied by publisher]
Source: Peritoneal Dialysis International - Category: Urology & Nephrology Tags: Perit Dial Int Source Type: research
François Chassagne1†, Xinyi Huang1†, James T. Lyles1 and Cassandra L. Quave1,2* 1Center for the Study of Human Health, Emory University, Atlanta, GA, United States 2Department of Dermatology, Emory University, Atlanta, GA, United States In the search for new therapeutic solutions to address an increasing number of multidrug-resistant bacterial pathogens, secondary metabolites from plants have proven to be a rich source of antimicrobial compounds. Ginkgo biloba, a tree native to China, has been spread around the world as an ornamental tree. Its seeds have been used as snacks and medical mater...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: 15 September 2017 Source:LWT - Food Science and Technology, Volume 83 Author(s): Surya Kant Verma, Shiv Kumar Sood, Ram Krishan Saini, Neha Saini The present study was carried out to enhance shelf life of raw buffalo milk by the use of pediocin PA-1 containing fermented cheese whey (PCFCW) as bio-preservative. Ten distinct genera of milk microbes could be identified as Streptococcus, Enterococcus, Salmonella, Campylobacter, Lactobacillus, Enterobacter, E. coli, Shigella, Candida and Aspergillus. For the production of PCFCW, supplemented cheese whey medium was fermented with Pediococcus pentosaceous NCDC ...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
The World Health Organization is issuing a warning about a group of deadly bacteria: Recently, the WHO released its first-ever list of “priority pathogens,” a list of antibiotic-resistant bacteria that the organization says pose the greatest threat to human health. The list is divided into three categories: critical-, high- and medium-priority. Three pathogens made it into the critical-priority group. These bacteria are resistant to multiple antibiotics and pose a high risk to people in hospitals and nursing homes, the WHO says. Multidrug-resistant bacteria, sometimes called “superbugs,” are a ...
Source: Science - The Huffington Post - Category: Science Source Type: news
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