GSE141513 Amino acid metabolism regulated by DNA Methylation in Mycobacterium bovis BCG str. Tokyo 172 induced by vitamins

Contributors : Ningning Song ; Guanghui Dang ; Ziyin Cui ; Huajun Zheng ; Siguo LiuSeries Type : Expression profiling by high throughput sequencingOrganism : Mycobacterium tuberculosis variant bovisVitamins are organic compounds and essential nutrients required by host organism. Recent studies have shown that vitamin B1(VB1) and vitamin C (Vc) could inhibit Mycobacterium tuberculosis growth. However, the precise role of VB1 and Vc in M.tuberculosis are still not well understand. Therefore, in this study, the transcriptional, metabolic and methylation profiles of VB1 and VC treated Mycobacterium. bovis BCG were analyzed by RNA-sequencing, LC-MS (Liquid chromatography coupled to mass spectrometry) and SMRT. The RNA-seq show that gene encoding cysteine synthase A was significantly up-regulated under both vitamins treatment, metabolic study show that most of amino acids decreased in VB1 treated BCG, in constrast, most of amino acids increased in VC treated samples. Methylome data show that m4c modificatiion partically is present in VC treated BCG and the expression of most genes harboring m4c methylation modificaiton increased. In all, these data imply that potentially increased concentration of cysteine might lead to BCG growth inhibition induced by VB1 and VC duing to produce superoxide which could cause DNA damage via Fetions reacition. The m4c methylation modification could promote gene transcription to some extent. In all, this study will benefit for us to futher understand ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mycobacterium tuberculosis variant bovis Source Type: research

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Source: Rural and Remote Health - Category: Rural Health Tags: Rural Remote Health Source Type: research
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Source: Journal of Ophthalmic Inflammation and Infection - Category: Opthalmology Source Type: research
Publication date: Available online 25 February 2020Source: European Journal of Integrative MedicineAuthor(s): Merlin Young, Jenny Craig
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Condition:   Tuberculosis Interventions:   Other: Access to Fast Track TB clinic;   Other: Information leaflet for male partner;   Other: Home sputum collection;   Other: Home HIV testing;   Other: Financial incentive Sponsors:   Liverpool School of Tropical Medicine;   Malawi-Liverpool-Wellcome Trust Clinical Research Programme;   London School of Hygiene and Tropical Medicine;   University of Liverpool Not yet recruiting
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Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
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Source: International Journal of Osteoarchaeology - Category: Science Authors: Tags: RESEARCH ARTICLE Source Type: research
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