Viruses, Vol. 12, Pages 108: In Vitro Hepatitis C Virus Infection and Hepatic Choline Metabolism

Viruses, Vol. 12, Pages 108: In Vitro Hepatitis C Virus Infection and Hepatic Choline Metabolism Viruses doi: 10.3390/v12010108 Authors: Kaelan Gobeil Odai Conor O’Dwyer Rineke Steenbergen Tyler A. Shaw Tyler M. Renner Peyman Ghorbani Mojgan Rezaaifar Shauna Han Marc-André Langlois Angela M. Crawley Rodney S. Russell John P. Pezacki D. Lorne Tyrrell Morgan D. Fullerton Choline is an essential nutrient required for normal neuronal and muscular development, as well as homeostatic regulation of hepatic metabolism. In the liver, choline is incorporated into the main eukaryotic phospholipid, phosphatidylcholine (PC), and can enter one-carbon metabolism via mitochondrial oxidation. Hepatitis C virus (HCV) is a hepatotropic positive-strand RNA virus that similar to other positive-strand RNA viruses and can impact phospholipid metabolism. In the current study we sought to interrogate if HCV modulates markers of choline metabolism following in vitro infection, while subsequently assessing if the inhibition of choline uptake and metabolism upon concurrent HCV infection alters viral replication and infectivity. Additionally, we assessed whether these parameters were consistent between cells cultured in fetal bovine serum (FBS) or human serum (HS), conditions known to differentially affect in vitro HCV infection. We observed that choline transport in FBS- and HS-cultured Huh7.5 cells is facilitated by the intermediate affinity transporter, choline tra...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research