Characterization of FosL1, a plasmid-encoded fosfomycin resistance protein identified in Escherichia coli.

Characterization of FosL1, a plasmid-encoded fosfomycin resistance protein identified in Escherichia coli. Antimicrob Agents Chemother. 2020 Jan 13;: Authors: Kieffer N, Poirel L, Descombes MC, Nordmann P Abstract Fosfomycin is gaining renewed interest for treating urinary tract infections. Monitoring fosfomycin resistance is therefore important in order to detect the emergence of novel resistance mechanisms. Here we used the Rapid Fosfomycin NP test to screen a collection of extended-spectrum ß-lactamase-producing Escherichia coli isolates from Switzerland and found a fosfomycin-resistant isolate in which a novel plasmid-mediated fosfomycin resistance gene, named fosL1, was identified. The FosL1 protein is a putative glutathione-S-transferase enzyme conferring high-level resistance to fosfomycin and sharing between 57 to 63 % amino-acid identity with other FosA-like family members. Genetic analyses showed that the fosL1 gene was embedded in a mobile insertion cassette and had likely been acquired by transposition through a Tn7-related mechanism. In-silico analysis over Genbank databases identified the FosL1 encoding gene in addition to another variant (fosL1-2) respectively in two Salmonella enterica isolates from the US. Our study further highlights the necessity of monitoring fosfomycin resistance in Enterobacteriaceae to identify the emergence of novel mechanisms of resistance. PMID: 31932373 [PubMed - as supplied by pub...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research