Substrate analogs that trap the 2'-phospho-ADP-ribosylated RNA intermediate of the Tpt1 (tRNA 2'-phosphotransferase) reaction pathway.

We report that replacing the NMN ribose of NAD+ with 2'-fluoroarabinose (thereby eliminating the ribose O2'' nucleophile) results in durable trapping of RNA-2'-phospho-(ADP-fluoroarabinose) as a "dead-end" product of step 1. Tpt1 enzymes from diverse taxa differ in their capacity to use ara-2''F-NAD+ as a substrate.
PMID: 31932322 [PubMed - as supplied by publisher]

Source: RNA - January 12, 2020 Category: Genetics & Stem Cells Authors: Dantuluri S, Abdullahu L, Munir A, Katolik A, Damha MJ, Shuman S Tags: RNA Source Type: research