Cutting Edge Approaches for Rapid Characterization of Airway Exosomes

Publication date: Available online 14 January 2020Source: MethodsAuthor(s): Kenneth P. Hough, Jessy S. DeshaneAbstractExosomes have been described as promising biomarkers for understanding disease progression and prognosis. These lipid membrane nanoparticles derived from airway cells have been shown to have immunomodulatory effects, such as driving inflammatory responses in asthma. These emerging evidences demonstrating an important pathophysiological role of exosomes warrants the development of novel approaches for isolation and rapid characterization of exosomes, which would be applicable for both translational and clinical studies. In this review article, we describe two methods of rapid exosomes characterization: (1) imaging flow cytometry using ImageStream; and (2) conventional flow cytometry using the BD Symphony A5 platform. We also explore sorting of exosomes using the BD Aria.Graphical abstractOverview of exosome characterization from either conditioned media or biological fluid, such as Broncho Alveolar Lavage (BAL) fluid, using both imaging and conventional flow cytometry.
Source: Methods - Category: Molecular Biology Source Type: research