Real-time redox monitoring of a nitrosyl ruthenium complex acting as NO-donor agent in a single A549 cancer cell with multiplex Fourier-transform infrared microscopy

Publication date: Available online 14 January 2020Source: Nitric OxideAuthor(s): Fernando Postalli Rodrigues, Lucyano J.A. Macedo, Leandro N.C. Máximo, Fernanda C.P.F. Sales, Roberto Santana da Silva, Frank N. CrespilhoAbstractMultiplex Fourier-transform infrared microscopy (μFT-IR) helped to monitor trans-[Ru(NO) (NH3)4 (isn)]3+ (I), uptake by A549 lung carcinoma cell, as well as the generation of its product, nitric oxide (NO), inside the cell. Chronoamperometry with NO-sensor and μFT-IR showed that exogenous NADH and the A549 cell induced the NO release redox mechanism. Chemical imaging confirmed that (I) was taken up by the cell, and that its localization coincided with its consumption in the cellular environment within 15 min of exposure. The Ru–NO absorption band in the IR spectrum shifted from 1,932 cm−1, when NO was coordinated to Ru such as {RuII–NO+}3+, to 1,876 cm−1, due the formation of reduced specie {RuII–NO0}2+, a precursor of NO release. Futhermore, the μFT-IR spectral profile demonstrated that, as a result of the No action on the target, NO interacted with nucleic acids, which provided a biochemical response that is detectable in living cells.
Source: Nitric Oxide - Category: Chemistry Source Type: research

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Abstract The purpose of the present study was to evaluate the clinical profiles and treatment outcomes of patients with lung cancer admitted to the Medical Psychiatric Unit (MPU), which is built for patients with physical and severe psychiatric disorders. All medical records of patients with lung cancer admitted to the MPU of Tachikawa hospital were reviewed. The clinical outcomes of these patients were retrospectively evaluated between January 2010 and December 2016. A total of 24 patients in the MPU were histologically or cytologically diagnosed with primary lung cancer. Of these, 20 patients had schizophrenia, ...
Source: Clinical Lung Cancer - Category: Cancer & Oncology Authors: Tags: Mol Clin Oncol Source Type: research
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Source: Biotechnology Reports - Category: Biotechnology Source Type: research
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Source: Journal of Investigative Surgery - Category: Surgery Tags: J Invest Surg Source Type: research
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Source: Recent Patents on Food, Nutrition and Agriculture - Category: Food Science Tags: Recent Pat Food Nutr Agric Source Type: research
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Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
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Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
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Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
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Source: Cancer Biomarkers - Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
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