Innate immunity is a late event in the onset of gliadin-specific enteropathy in the HLA-DQ8 mice.

Innate immunity is a late event in the onset of gliadin-specific enteropathy in the HLA-DQ8 mice. Immunobiology. 2020 Jan 08;:151903 Authors: Maurano F, Ogita T, Luongo D, Rotondi Aufiero V, Bergamo P, Mazzarella G, Tanabe S, Rossi M Abstract Celiac disease (CD) is a food enteropathy that occurs in genetically susceptible individuals following the ingestion of gluten. Both gluten cytotoxicity and immunity activation play a role in CD pathogenesis; however, the chronological assessment of the different pathogenic mechanisms remains elusive. The models developed so far have only partially addressed this issue. Herein, Ab°DQ8 transgenic mice were administered wheat gliadin and indomethacin for 10 days to induce enteropathy. Gliadin-induced alteration of the small intestinal architecture was associated with increased expression of tissue transglutaminase in the lamina propria and a marked hypoxic environment. Enteropathic mice showed activation of innate immunity, featuring an increase of pro-inflammatory IFN-γ and IL-15 mRNAs, as well as CD11c+CD103+, CD11b+CD11c+, and CD11b+CD103+ dendritic cell subsets. However, the temporal assessment of examined parameters indicated that the induction of innate immunity during the generation of the mucosal lesion, occurred belatedly, highlighting a major role of gliadin intrinsic cytotoxicity in the pathogenic mechanism of this model. These results have important implications for the use of this m...
Source: Immunobiology - Category: Allergy & Immunology Authors: Tags: Immunobiology Source Type: research