Distribution of microbial communities and core microbiome in successive wound grades of diabetic foot ulcer individuals.

Distribution of microbial communities and core microbiome in successive wound grades of diabetic foot ulcer individuals. Appl Environ Microbiol. 2020 Jan 10;: Authors: Jnana A, Muthuraman V, Varghese VK, Chakrabarty S, Murali TS, Ramachandra L, Shenoy KR, Rodrigues GS, Prasad SS, Dendukuri D, Morschhauser A, Nestler J, Peter H, Bier FF, Satyamoorthy K Abstract Diabetic foot ulcer (DFU) is a major complication of diabetes with high morbidity and mortality rates. Pathogenesis of DFUs is governed by a complex milieu of environmental and host factors. The empirical treatment is initially based on wound severity since culturing and profiling the antibiotic sensitivity of wound-associated microbes is time consuming. Hence, a thorough and rapid analysis of the microbial landscape is a major requirement towards devising evidence-based interventions. Towards this, 122 wound (100 diabetic and 22 non-diabetic) samples were sampled for their bacterial community structure using both culture-based and next-generation 16S rRNA based metagenomics approach. Both the approaches showed that the Gram-negative microbes were more abundant in the wound microbiome. The core microbiome consisted of bacterial genera including Alcaligenes, Pseudomonas, Burkholderia, and Corynebacterium in decreasing order of average relative abundance. Despite the heterogenous nature and extensive sharing of microbes, an inherent community structure was apparent as revealed by...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research