Fast, robust and effective decellularization of whole human livers using mild detergents and pressure controlled perfusion.

In conclusion, we established effective, robust and fast decellularization protocols for both porcine and human livers. With this protocol the duration of decellularization for whole-organ human livers has been shortened considerably. The increased pressure and flow did not damage the ECM, as major ECM components remained intact.
PMID: 31923991 [PubMed - in process]

Source: Appl Human Sci - January 13, 2020 Category: Physiology Authors: Willemse J, Verstegen MMA, Vermeulen A, Schurink IJ, Roest HP, van der Laan LJW, de Jonge J Tags: Mater Sci Eng C Mater Biol Appl Source Type: research