CRISPR/Cas9 gene editing in a chicken model: current approaches and applications.

CRISPR/Cas9 gene editing in a chicken model: current approaches and applications.
J Appl Genet. 2020 Jan 10;:
Authors: Chojnacka-Puchta L, Sawicka D
Abstract
Improvements in genome editing technology in birds using primordial germ cells (PGCs) have made the development of innovative era genome-edited avian models possible, including specific chicken bioreactors, production of knock-in/out chickens, low-allergenicity eggs, and disease-resistance models. New strategies, including CRISPR/Cas9, have made gene editing easy and highly efficient in comparison to the well-known process of homologous recombination. The clustered regularly interspaced short palindromic repeats (CRISPR) technique enables us to understand the function of genes and/or to modify the animal phenotype to fit a specific scientific or production target. To facilitate chicken genome engineering applications, we present a concise description of the method and current application of the CRISPR/Cas9 system in chickens. Different strategies for delivering sgRNAs and the Cas9 protein, we also present extensively. Furthermore, we describe a new gesicle technology as a way to deliver Cas9/sgRNA complexes into target cells, and we discuss the advantages and describe basal applications of the CRISPR/Cas9 system in a chicken model.
PMID: 31925767 [PubMed - as supplied by publisher]

Source: J Appl Genet - January 9, 2020 Category: Genetics & Stem Cells Authors: Chojnacka-Puchta L, Sawicka D Tags: J Appl Genet Source Type: research