Pharmacokinetic Effects of ginsenoside Rg1 on Aconitine, Benzoylaconine and Aconine by UHPLC-MS/MS.

In this study, the ginsenoside Rg1 was simultaneously intragastrically administered to rats with AC, BAC and ACN respectively, and the rat plasma was collected at different time points. The plasma drug concentrations of three types of alkaloids were determined by UHPLC-MS/MS, and the pharmacokinetic parameters were calculated. The results indicated that the Cmax and AUC(0-t) of BAC were significantly increased (p<0.05), AC was decreased (p<0.05), and ACN did not changed after pretreatment of ginsenoside Rg1. It was inferred that ginsenoside Rg1 may affect the absorption and metabolism of AC and BAC and then change their pharmacokinetic parameters. Subsequently, their absorption and metabolism were further investigated by using the Caco-2 cell monolayer and rat liver microsomes in vitro. The Caco-2 cell monolayer absorption assay indicated that ginsenoside Rg1 could promote the absorption of AC and BAC, and the rat liver microsomes metabolism assay indicated that ginsenoside Rg1 accelerated the metabolism of AC and did not affect the other two alkaloids. All the results indicated that ginsenoside Rg1 may reduce the toxicity of aconite and improve its efficacy by promoting the absorption of BAC and accelerating the metabolism of AC. These results could provide evidences for compatibility mechanism of the traditional Chinese herbal formula Shenfu Decoction. PMID: 31919877 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research