A novel low virulent respiratory infectious bronchitis virus originating from the recombination of QX, TW and 4/91 genotype strains in China

Publication date: Available online 11 January 2020Source: Veterinary MicrobiologyAuthor(s): Mengjiao Huang, Chuangchao Zou, Yuan Liu, Zhenling Han, Chunyi Xue, Yongchang CaoAbstractIn China, variants of infectious bronchitis virus (IBV) evolve continually and diverse recombinant strains have been reported. Here, an IBV strain, designated as ck/CH/LJX/2017/07 (referred as JX17) was isolated from chicken vaccinated with H120 and 4/91 in Jiangxi, China, in 2017. Sequence analysis reveals of the S1 gene of JX17 the highest nucleotide identity of 98.15% with that of GI-7 genotype TW2575/98 strain. Furthermore, whole genome analysis among JX17 and other 18 IBV strains demonstrates that JX17 has the highest nucleotide identity of 95.94% with GI-19 genotype YX10 strain. Among all genes of JX17 except the S1 gene, the N gene and 3’ UTR have the highest identity to GI-13 genotype 4/91 strain and the rest genes are the most identical to GI-19 genotype YX10 strain. Analyzed by the RDP and SimPlot, the recombination of JX17 strain was shown to occur in regions which include 5’-terminal S1 gene (20,344 to 22,447 nt), most N gene and 3’ UTR (26,163 to 27,648 nt). The pathogenicity study shows that JX17 is a natural low virulent IBV variant which caused respiratory symptoms but no death. Taken together, these results indicate that IBV strains continue to evolve through genetic recombination and three prevalent genotypes in China including QX, TW ...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research

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Publication date: April 2020Source: Molecular Immunology, Volume 120Author(s): Bal Krishan Sharma, Naresh Kumar Kakker, Sakshi Bhadouriya, Rajesh ChhabraAbstractAvian infectious bronchitis (IB) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (IBV) belonging to the genus Coronavirus and family Coronaviridae. It can affect all age groups of birds. The toll-like receptors (TLRs) are a major class of innate immune pattern recognition receptors that have a key role in immune response and defense against various infections.The TLRs are essential for initiation o...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
In this study, we constructed the recombinant strain, rH120-S1/YZ, using a reverse genetic system, based on the backbone of the H120 vaccine strain, with the S1 gene replaced with that of the QX-like nephropathogenic strain, ck/CH/IBYZ/2011, isolated in China. The results of dwarf chicken embryos, growth kinetics, and viral titration in the embryos demonstrated that the biological characteristics of the recombinant virus remained unchanged. Like the rH120-infected group and in contrast to the rIBYZ-infected group, no mortality, clinical signs, or lesions were observed in the lungs or kidneys of young chickens inoculated wi...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
RARITAN, NJ, February 10, 2020 – The Janssen Pharmaceutical Companies of Johnson &Johnson announced today the submission of a supplemental Biologics License Application (sBLA) to the U.S. Food and Drug Administration (FDA) seeking approval of DARZALEX® (daratumumab) in combination with Kyprolis® (carfilzomib) and dexamethasone (DKd) for relapsed/refractory multiple myeloma. The sBLA is supported by results from the Phase 3 CANDOR study, which compared treatment with DKd to carfilzomib and dexamethasone (Kd) in patients with multiple myeloma who relapsed after one to three prior lines of therapy. “Wh...
Source: Johnson and Johnson - Category: Pharmaceuticals Tags: Innovation Source Type: news
Abstract INTRODUCTION: Influenza vaccination is the most effective way to prevent influenza. Few studies on its rate were reported throughout China and for populations with chronic diseases. An estimation of the rates in China was accomplished. METHODS: All data were from a national cross-sectional survey of a sample representing the population aged 40 years or older in mainland China in 2014-15. A total of 74,484 individuals with complete self-reported influenza vaccination status were analyzed in 2018-19. RESULTS: The overall influenza vaccination rate was 2.4% (95% CI 1.4-3.3) with 1.7% (95% CI 1...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
A rapidly evolving health story broke in late December when a novel illness originating in Wuhan, China made the news. Reports of the number of infected people swiftly rose, and isolated cases of this new coronavirus — dubbed 2019-nCoV by scientists — have appeared in several countries due to international travel. At this writing, almost 1,300 confirmed cases and over 40 deaths have occurred in China, according to an article in the New York Times. Fortunately, public health officials in many countries, including the US, have put measures in place to help prevent further spread of the virus. These measures inclu...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Health Source Type: blogs
What is a coronavirus? Coronaviruses are a group of viruses that cause diseases in mammals, including humans, and birds. Why are they called coronaviruses? The name derives from the fact that the viral capsule has a “halo” or “crown” surrounding it. What do coronaviruses do? In humans, the virus infects the airways giving rise to flu-like symptoms, a runny nose, cough, sore throat and fever, these are usually mild, but in rare cases can be lethal. Is there a vaccine against coronaviruses? No. Are there any drugs to block or treat infection? No. When were coronaviruses first discovered? In the 1960s ...
Source: David Bradley Sciencebase - Songs, Snaps, Science - Category: Science Authors: Tags: Sciencebase Source Type: blogs
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): A. Laconi, E. Weerts, J. Bloodgood, J. Déniz, A. Berends, G. Cocciolo, S. de Wit, H. Verheije
Source: Journal of Comparative Pathology - Category: Pathology Source Type: research
Wu Cui Cao Huang The QX-type avian infectious bronchitis virus (IBV) is still a prevalent genotype in Southwestern China. To analyze the antigenicity and pathogenicity characteristics of the dominant genotype strains (QX-type), S1 gene sequence analysis, virus cross-neutralization tests, and pathogenicity test of eight QX-type IBV isolates were conducted. Sequence analysis showed that the nucleotide homology between the eight strains was high, but distantly related to H120 and 4/91 vaccine strains. Cross-neutralization tests showed that all eight strains isolated from 2015 and 2017 belonged to the same sero...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
CONCLUSIONS Our results showed there was a seasonal measles outbreak in Shenzhen Children's Hospital. All the measles virus from 129 measles patients were H1 viruses. The clinical signs also showed a difference between unvaccinated and vaccinated patients. Moreover, most of the unvaccinated patients came from migrant worker families. We suggest there is a need for increased health promotion and vaccination programs for migrant workers and people living in remote villages. PMID: 31800568 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Abstract The use of live attenuated vaccine (LAV) is the main method for controlling infectious bronchitis (IB). It is advisable to develop a LAV using a dominant serotype in the region in the case of vaccine failure. Since 793/B serotype is one of the most predominant circulating IB viruses in Iran, attenuation of three Iranian 793/B isolates (IR/773/2001, IR/794/2002 and IR/520/2002) was done by serial passaging in specific pathogen free (SPF) embryonated chicken eggs up to 90 passages to assess the degree of their attenuation to achieve a native LAV in the future. Virulence and pathogenicity of passage levels 1...
Source: Acta Virologica - Category: Infectious Diseases Authors: Tags: Acta Virol Source Type: research
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