Direct detection of Listeria monocytogenes DNA amplification products with quartz crystal microbalances at elevated temperatures

Publication date: Available online 7 January 2020Source: Sensors and Actuators B: ChemicalAuthor(s): Sirirat Wachiralurpan, Kosum Chansiri, Peter A. LieberzeitAbstractCurrent methods for identifying Listeria monocytogenes are both time and labor intensive. However, it is highly desirable to detect it rapidly and reliably to prevent and/or identify contamination of foodstuff. Herein we propose a mass-sensitive sensor operating at elevated temperatures, 50-55 °C, for that purpose. Sensitive and selective detection relies on distinguishing genes of genomic extract of L. monocytogenes. A thiol-modified ssDNA probe designed for virulence phosphatidylcholine-phospholipase C (plcB) immobilized on the Quartz Crystal Microbalance (QCM) serves as the recognition element. This hybridizes with synthetic Loop-mediated isothermal amplification (LAMP) products of target DNA on the active surface sensor. Discernible detection limits of approximately 3 × 10-1 to 3 × 10 °CFU mL-1 of L. monocytogenes DMST 17303 gDNA were achieved. The QCM DNA sensor showed high sensitivity and selectivity for L. monocytogenes (100%) with negligible interference by DNA of other food-borne pathogens, such as Salmonella Paratyphi A (24%), Salmonella Weltevreden (24%), Salmonella Typhi (16%), Shigella boydii (22%), and Shigella flexneri (13%). The temperature covered is in the range of 50 to 55 °C for immobilizing DNA probe and DNA targeted hy...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research

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A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens. J Food Sci. 2020 Jan 30;: Authors: Tao J, Liu W, Ding W, Han R, Shen Q, Xia Y, Zhang Y, Sun W Abstract Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time-consuming and labor-intensive. Mult...
Source: Anal Sci - Category: Chemistry Authors: Tags: J Food Sci Source Type: research
In this study, we established a whole-cell method for the identification of foodborne pathogenic bacteria, using MALDI-TOF MS and principal component analysis (PCA), which did not use protein extractions or expensive protein databases. Thirty strains comprising six common foodborne pathogenic bacteria, namely, Shigella flexneri, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Pseudomonas aeruginosa, and Listeria monocytogenes were analyzed using MALDI-TOF MS. The culture time, matrix, and spotting method were optimized based on peak intensity and deviation. A PCA was performed to analyze the mass spectrome...
Source: Analytical Biochemistry - Category: Biochemistry Authors: Tags: Anal Biochem Source Type: research
Publication date: 15 March 2020Source: Journal of Molecular Structure, Volume 1204Author(s): Duygu Åžahin Gül, Hatice Ogutcu, Zeliha HayvalıAbstractTwo different series of crown ether compounds (4-11) were synthesized by the reactions of 4′,5′-bis(bromomethyl)benzo-15-crown-5 (3) and 4′-amino-benzo-15-crown-5 with hydroxycoumarin and chromone derivatives. Coumarin-crown ether compounds (4 and 5) were synthesized by the reactions of 4′,5′-bis(bromomethyl)benzo-15-crown-5 (3) with 4-hydroxycoumarin and 7-hydroxycoumarin. Chromone-crown ether compounds (6-11), were synthesized by the conden...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
koh Disease outbreaks caused by the ingestion of contaminated vegetables and fruits pose a significant problem to human health. The sources of contamination of these food products at the preharvest level of agricultural production, most importantly, agricultural soil and irrigation water, serve as potential reservoirs of some clinically significant foodborne pathogenic bacteria. These clinically important bacteria include: Klebsiella spp., Salmonella spp., Citrobacter spp., Shigella spp., Enterobacter spp., Listeria monocytogenes and pathogenic E. coli (and E. coli O157:H7) all of which have the potential to cause dise...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
Updated Date: Fri, 13 Sep 2019 00:00:00 EDT
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
Publication date: Available online 10 June 2019Source: LWTAuthor(s): Sujogya Kumar Panda, Yugal Kishore Mohanta, Laxmipriya Padhi, Walter LuytenAbstractThe anti-microbial activity of crude extracts (hexane, acetone, ethanol, and aqueous) of 46 edible plants from Odisha, India, was studied using a broth microdilution assay against 8 common food-borne pathogens (Gram-positive: Bacillus cereus Streptococcus faecalis, Listeria innocua, Micrococcus luteus, and Gram-negative: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Shigella sonnei). In total, 46 plant species belonging to 31 families were tested, from whic...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Gulcin Tezcan1, Ekaterina V. Martynova1, Zarema E. Gilazieva1, Alan McIntyre2, Albert A. Rizvanov1 and Svetlana F. Khaiboullina1,3* 1Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia 2Centre for Cancer Sciences, Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham, United Kingdom 3Department of Microbiology and Immunology, University of Nevada, Reno, Reno, NV, United States Inflammation has a crucial role in protection against various pathogens. The inflammasome is an intracellular multiprotein signaling complex that is linked to pathogen sensing and...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Conclusion This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the viability of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
François Chassagne1†, Xinyi Huang1†, James T. Lyles1 and Cassandra L. Quave1,2* 1Center for the Study of Human Health, Emory University, Atlanta, GA, United States 2Department of Dermatology, Emory University, Atlanta, GA, United States In the search for new therapeutic solutions to address an increasing number of multidrug-resistant bacterial pathogens, secondary metabolites from plants have proven to be a rich source of antimicrobial compounds. Ginkgo biloba, a tree native to China, has been spread around the world as an ornamental tree. Its seeds have been used as snacks and medical mater...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Discussion Until now, the main tool used for bacterial identification based on NGS of the 16S–23S rRNA encoding region was de novo assembly followed by BLASTN on NCBI database (Sabat et al., 2017), however, there was no evidence that it would be the most accurate and/or fastest method available. The de novo assembly and BLASTN is the only approach of the three that works at the contig level, and both the OTU clustering and mapping are performed at the read level. Using the NCBI database for these two last approaches would have resulted in odd results, since the NCBI database includes sequences that do not belong to ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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