The Escherichia coli RnlA – RnlB toxin – antitoxin complex: production, characterization and crystallization

The Escherichia coli rnlAB operon encodes a toxin – antitoxin module that is involved in protection against infection by bacteriophage T4. The full-length RnlA – RnlB toxin – antitoxin complex as well as the toxin RnlA were purified to homogeneity and crystallized. When the affinity tag is placed on RnlA, RnlB is largely lost during purification and the resulting crystals exclusively comprise RnlA. A homogeneous preparation of RnlA – RnlB containing stoichiometric amounts of both proteins could only be obtained using a His tag placed C-terminal to RnlB. Native mass spectrometry and SAXS indicate a 1:1 stoichiometry for this RnlA – RnlB complex. Crystals of the RnlA – RnlB complex belonged to space group C2, with unit-cell parameters a = 243.32, b   =   133.58, c = 55.64   Å , β = 95.11 ° , and diffracted to 2.6   Å resolution. The presence of both proteins in the crystals was confirmed and the asymmetric unit is likely to contain a heterotetramer with RnlA2:RnlB2 stoichiometry.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: toxin – antitoxin bacterial stress response macromolecular complex Escherichia coli research communications Source Type: research

Related Links:

In conclusion, our results suggested that AVNP2 could have an effect on the peri-tumor environment, obviously restraining the growth progress of gliomas, and eventually improving cognitive levels in C6-bearing rats.
Source: Brain, Behavior, and Immunity - Category: Neurology Source Type: research
ConclusionThe probability of virus detection is independent of the time between notification of the outbreak or symptom onset and sample collection. Our results suggest possible defects in cleaning protocols and disinfection in closed and semi-closed settings.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Estimates of acute gastroenteritis (AGE) burden are difficult to compare between studies because of inconsistent definitions describing this illness. AGE definitions used in prospective, community-based childhood cohort studies were identified by searching databases for studies that collected daily observations of AGE symptoms. Disease definitions and refractory periods were extracted. Data from the Australian community-based Observational Research in Childhood Infectious Diseases birth cohort were used to calculate AGE incidence and duration using identified AGE definitions, and the World Health Organization definition fo...
Source: Journal of Pediatric Gastroenterology and Nutrition - Category: Gastroenterology Tags: Short Communications: Gastroenterology Source Type: research
We examined the nutritional composition and chemical resilience of fish roe skeins from the flying fish (Hirundichthys oxycephalus) that are commonly found in temperate and tropical oceans. The air-dried skeins contained 72-74% crude protein, high-quality protein composed of essential and most non-essential amino acids, and very low carbohydrate content. High macro and micro minerals, lipids, albeit at a low amount, composed of saturated and unsaturated fatty acids and water- and lipid-soluble vitamins were also present. The roe skeins were highly durable and resistant to nutrient decomposition through over-desiccation as ...
Source: Journal of Food Composition and Analysis - Category: Food Science Source Type: research
Conclusion: Escherichia coli, the predominant uropathogen, showed significant multidrug resistance to antibiotics commonly prescribed for the management of UTIs. These findings should form a basis for preliminary decision making on the appropriate line of treatment for UTIs.Escherichia coli isolates were highly susceptible to cefotaxime/clavulanic acid (100%) and nitrofurantoin (70%) but showed high resistance to cefuroxime (100%), ceftazidime (100%), nalidixic acid (90%), and ciprofloxacin (90%). PMID: 32076437 [PubMed]
Source: Interdisciplinary Perspectives on Infectious Diseases - Category: Infectious Diseases Tags: Interdiscip Perspect Infect Dis Source Type: research
Publication date: Available online 21 February 2020Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Khamis Al-Dhafri, Chai Lay Ching, Saiful Anuar KarsaniAbstractThe emergence of antibiotic resistance among bacteria leads to the urge of finding an alternative solution. Therefore, antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. This research was designed to explore the potency of antimicrobial peptides from Omani Juniperus seravschanica plant and their mode of action. The isolated AMPs showed to have inhibitory activity towards pathog...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
Authors: Blakiston MR, Freeman JT Abstract AIMS: National responses to antimicrobial resistance (AMR) require an understanding of the factors driving its development and spread. Research to date has primarily focused on determining individual-level risk factors for AMR-associated infections. However, additional insights may be gained by investigating exposures associated with AMR variation at the population level. METHODS: We used an ecological study design to describe the association between the incidence rate of methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase producin...
Source: New Zealand Medical Journal - Category: General Medicine Tags: N Z Med J Source Type: research
Publication date: Available online 20 February 2020Source: Microbial PathogenesisAuthor(s): Ying Liu, Xi Tan, Haojin Cheng, Jie Gong, Yong Zhang, Daibin Wang, Wei DingAbstractCold shock proteins (Csps) are small and highly conserved proteins that have target RNA- and DNA-binding activities. Csps play roles in different cellular processes and show functional redundancy. Ralstonia solanacearum, the agent of bacterial wilt, has 4 or 5 Csps based on genome analysis. However, the functions of all Csps in R. solanacearum remain unclear. According to phylogenetic analysis, the Csps from R. solanacearum are clustered into a group ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
CONCLUSIONS The results of our study suggest that the method of implant decontamination should be customized to the type of implant surface. PMID: 32078588 [PubMed - as supplied by publisher]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Authors: Akter S, Huq MA Abstract The present study highlights the biological synthesis of silver nanoparticles (AgNPs) using Sphingobium sp. MAH-11 and also their antibacterial mechanisms against drug-resistant pathogenic microorganisms. The nanoparticle synthesis method used in this study was reliable, facile, rapid, cost-effective and ecofriendly. The AgNPs exhibited the highest absorbance at 423 nm. The TEM image expressed spherical shape of AgNPs and the size of synthesized AgNPs was 7-22 nm. The selected area diffraction (SAED) pattern and XRD spectrum revealed the crystalline structure of AgNPs...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
More News: Biochemistry | Gastroenteritis