Antimicrobial Activity and Biocompatibility of Slow-release Hyaluronic Acid-antibiotic Conjugated Particles

Publication date: Available online 9 January 2020Source: International Journal of PharmaceuticsAuthor(s): Ze Zhang, Selin S. Suner, Diane A. Blake, Ramesh S. Ayyala, Nurettin SahinerAbstractHere, the aim was to design and use a long-lasting antibiotic release system for prevention of postoperative infections in ophthalmic surgery. Ciprofloxacin and vancomycin-conjugated hyaluronic acid (HA) particles were prepared as drug carriers for sustained release of antibiotics. The antimicrobial effects of the released drugs were determined by disc-diffusion and macro-dilution tests at different times up to 2 weeks. Slow degradable HA particles were obtained with 35.2 wt% degradation within 21 days. The drug loading amount was increased by employing two sequential chemical linking (conjugation, 2C) and one physical absorption loading (A) procedures (2C+A processes) from 148±8 to 355±11 mg/g HA particles for vancomycin. The amounts of vancomycin and ciprofloxacin that were released linearly was estimated as 64.35±7.35 and 25.00±0.68 mg/g, respectively, from drug-conjugated HA particles in 100 hours. Antimicrobial studies revealed that antibiotic-conjugated HA particles could inhibit the growth of microorganisms from 1 hour to 1 week. The MBC values were measured as 0.25, 4.0, and 0.25 mg/mL against Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis, respectively, after 72 hours incubation time. Cytotoxicity studies showed no difference between fibroblast growth or ...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research