Immunohistochemical evaluation of tumor budding in colorectal cancer: an important parameter with prognostic value.

Immunohistochemical evaluation of tumor budding in colorectal cancer: an important parameter with prognostic value. Rom J Morphol Embryol. 2019;60(3):841-846 Authors: Meşină C, Stoean CL, Stoean R, Săndiţă AV, Dumitrescu TV, Mogoantă SŞ, Cristian DA, Meşină-Botoran MI, Mitroi G, Gruia CL, Foarfă MC, Meşină M, Ciobanu D Abstract We analyzed 82 patients with colorectal cancer (CRC) [75 patients with mucinous adenocarcinoma (ADK) and seven patients with "signet ring cell" ADK] using multi-cytokeratin (CK) AE1∕AE3 immunohistochemical assay. In order to determine the mucinous nature of some of the lymph node metastases of the mucinous colorectal ADKs studied, Periodic Acid Schiff-Alcian Blue (PAS-AB) histochemical staining was used. The counting results were systematized in the following ranges: 0 budding areas; between 1-4 budding areas; between 5-9 budding areas; and =10 tumor budding (TB) areas. The statistical analysis was performed using the Student's t-test. More than half of the cases of mucinous ADK revealed an increased intensity of TB, whereas in the case of "signet ring cell" ADK, an average intensity of this phenomenon. Mucinous ADKs, which were pT3 staged, showed an increased intensity of TB, and those in pT2 stage demonstrated, in the vast majority of cases, the absence of TB. There was a predominance of TB intensity in the absence of vascular-lymphatic invasion. Our study shows the existence of a concordance b...
Source: Romanian Journal of Morphology and Embryology - Category: General Medicine Tags: Rom J Morphol Embryol Source Type: research